We narrowed to 5,897 results for: pcDNA 3.1

1. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

   Plasmid

   #124494

   Purpose

   Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Caspase-1 cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124428

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

   Plasmid

   #188991

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   F163L, K249R, G328S, G381V, G429D, D480G

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

   Plasmid

   #188992

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K109R, P245A, G328S, G381V, G429D

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

   Plasmid

   #188993

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   D201G, A258T, K327R, G328S, G381V, G429D, T450A

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124429

   Purpose

   Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(TEV cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

   Plasmid

   #188994

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5'-BirA-FLAG Sap30bp-exon minimal CMV pCDNA5

   Plasmid

   #212092

   Purpose

   LR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Sap30bp-exon (Sap30bp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5'-BirA-FLAG Sap30bp+exon minimal CMV pCDNA5

    Plasmid

    #212093

    Purpose

    LR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Sap30bp+exon(48nt) (Sap30bp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5'-BirA-FLAG Chtop-exon minimal CMV pCDNA5

    Plasmid

    #212090

    Purpose

    LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop-exon (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

    Plasmid

    #212091

    Purpose

    LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop+exon(138nt) (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2-Cx43-M100/125/147/213-siResist

    Plasmid

    #49858

    Purpose

    Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100, Methionine 125, Methionine 147, an…

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. 5'-BirA-FLAG Kdm1a-exon minimal CMV pCDNA5

    Plasmid

    #212088

    Purpose

    LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a-exon (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5

    Plasmid

    #212089

    Purpose

    LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a+exon(12nt) (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

    Plasmid

    #212818

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    CUbo(K48R/K63R)-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113611

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    mu opioid receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113610

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    beta 2 adrenergic receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113608

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    PAR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge