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EF1A_Hygro_P2A_RUNX1_R64P_Barcode

Plasmid

#170927

Purpose

Overexpression of RUNX1 R64P mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R64P

Promoter

Ef1A

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_T65I_Barcode

Plasmid

#170928

Purpose

Overexpression of RUNX1 T65I mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T65I

Promoter

Ef1A

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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NarCas9_AAV

Plasmid

#192145

Purpose

Expresses NarCas9?and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

NarCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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Portal_prophage_Desulfurococcales_archaeon

Plasmid

#188816

Purpose

Portal protein from Desulfurococcales archaeon ex4484_217_2 prophage

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Portal

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Terminase_prophage_Desulfurococcales_archaeon

Plasmid

#188841

Purpose

Large terminase from Desulfurococcales archaeon ex4484_217_2 prophage

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Terminase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF8_P2A_Hygro_Barcode

Plasmid

#120494

Purpose

Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF8 (KLF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac(UV5)

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_DARC_p.W253R

Plasmid

#82749

Purpose

Gateway Donor vector containing DARC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

W253R; S326F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DARC_p.T211S

Plasmid

#82750

Purpose

Gateway Donor vector containing DARC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACKR1 (ACKR1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T211S; S326F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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WT Smarca4-sfGFP

Plasmid

#107056

Purpose

Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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