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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AGG1984 UbL40-Bs

Plasmid

#242439

Purpose

Ae aegypti UbL40 promoter expressing barstar

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barstar

Expression

Insect

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB605-GBSSI

Plasmid

#213867

Purpose

Binary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Expression

Bacterial and Plant

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB560-GBSSI

Plasmid

#213866

Purpose

Binary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Expression

Bacterial and Plant

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pTCB-nivoLC

Plasmid

#237378

Purpose

pTwist CMV BetaGlobin encoding nivolumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

nivolumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-pembroLC

Plasmid

#237380

Purpose

pTwist CMV BetaGlobin encoding pembrolizumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

pembrolizumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelLC

Plasmid

#237384

Purpose

pTwist CMV BetaGlobin encoding avelumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

avelumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-rituxLC

Plasmid

#237372

Purpose

pTwist CMV BetaGlobin encoding rituximab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

rituximab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-cetuxLC

Plasmid

#237374

Purpose

pTwist CMV BetaGlobin encoding cetuximab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

cetuximab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroLC

Plasmid

#237376

Purpose

pTwist CMV BetaGlobin encoding magrolimab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

magrolimab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-ER [TM(SAC1)]

Plasmid

#186475

Purpose

Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTCB-nivoHCLALAPGknobHA

Plasmid

#237377

Purpose

pTwist CMV BetaGlobin encoding nivolumab heavy chain with LALA P329G knob Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

nivolumab heavy chain (silent Fc)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-pembroHCLALAPGknobHA

Plasmid

#237379

Purpose

pTwist CMV BetaGlobin encoding pembrolizumab heavy chain with LALA P329G knob Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

pembrolizumab heavy chain (silent Fc)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelHCknob6xHis

Plasmid

#237383

Purpose

pTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

avelumab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-trasHCknob6xHis

Plasmid

#237364

Purpose

pTwist CMV BetaGlobin encoding trastuzumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

trastuzumab heavy chain

Use

Affinity Reagent/ Antibody

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-trasLC

Plasmid

#237365

Purpose

pTwist CMV BetaGlobin encoding trastuzumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

trastuzumab light chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-Sig9R120AFcholeHA

Plasmid

#237369

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc R120A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-9-Fc (R120A mutant) (SIGLEC9 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-rituxHCknob6xHis

Plasmid

#237371

Purpose

pTwist CMV BetaGlobin encoding rituximab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

rituximab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-cetuxHCknob6xHis

Plasmid

#237373

Purpose

pTwist CMV BetaGlobin encoding cetuximab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

cetuximab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig7FcholeHA

Plasmid

#237382

Purpose

pTwist CMV BetaGlobin encoding Siglec-7-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-7-Fc (SIGLEC7 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TCB-Sig7FcLALAPGholeHA

Plasmid

#237366

Purpose

pTwist CMV BetaGlobin encoding Siglec-7-Fc with LALA P329G hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-7-silentFc (SIGLEC7 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig9FcholeHA

Plasmid

#237367

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-9-Fc (SIGLEC9 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-Sig10FcholeHA

Plasmid

#237370

Purpose

pTwist CMV BetaGlobin encoding Siglec-10-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-10-Fc (SIGLEC10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-Sig7R124AFcholeHA

Plasmid

#237368

Purpose

pTwist CMV BetaGlobin encoding Siglec-7-Fc R124A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-7-Fc (R124A mutant) (SIGLEC7 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCB-Gal9NFcLALAPGhole6xHis

Plasmid

#237381

Purpose

pTwist CMV BetaGlobin encoding Galectin-9 N terminal carbohydrate binding domain-Fc fusion with LALA P329G hole Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Galectin-9-silentFc (N-terminal carbohydrate binding domain only) (LGALS9 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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