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Addgene

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We narrowed to 1,239 results for: membrane gfp

Showing: 601 - 640 of 1239 results

  4. pLenti-CMV-paGFP-bPAC-CAAX

    Plasmid
    #204671
    Purpose
    Mammalian expression of membrane-bound bPAC tagged with paGFP for cAMP production
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Insert
    paGFP-bPAC-CAAX
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204671-plasmid-map-sequence-id-407793

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  6. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  14. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

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  33. EGFP–COSMO–PB

    Plasmid
    #169684
    Purpose
    COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104W
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169684-plasmid-map-sequence-id-338138

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  34. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody
    Use
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169685-plasmid-map-sequence-id-338140

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  35. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    David Andrews
    Insert
    Bcl-2-interacting killer (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191411-plasmid-map-sequence-id-376884

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  37. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid
    #163917
    Purpose
    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers
    Depositor
    Markus Affolter
    Article
    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do
    Insert
    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163917-plasmid-map-sequence-id-323453

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Showing: 601 - 640 of 1239 results