We narrowed to 1,308 results for: G3

1. pSDH52

   Plasmid

   #139049

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (includes STOP codon)
   

   Expression

   Worm

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJS011

   Plasmid

   #188334

   Purpose

   PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::mTagBFP2::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ173

   Plasmid

   #98410

   Purpose

   Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
   

   Use

   CRISPR

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJS008

   Plasmid

   #188331

   Purpose

   PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::wrmScarlet::mIAA7
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-TarHom-Tol2-kanaR-cryaa-dsRed

   Plasmid

   #74152

   Purpose

   BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   Tol2-kanaR-cryaa-dsRed
   

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-his6-HA3-SNAPf

   Plasmid

   #188941

   Purpose

   T7 expression vector for making his6- and HA3-tagged SNAP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-SNAPf
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJS006

   Plasmid

   #188329

   Purpose

   PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::mNeonGreen::mIAA7
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHACK(Gal4)-DONR(T2A-Flp1)

   Plasmid

   #194770

   Purpose

   To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

   Depositor

   Chun Han

   Article

   Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

   Insert

   T2A-Flp1
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB4282

   Plasmid

   #98701

   Purpose

   A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   gRNA PCR template
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJS005

    Plasmid

    #188328

    Purpose

    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::mNeonGreen::Linker
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJF6

    Plasmid

    #139040

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (includes STOP codon, PATC introns)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-SKII-3XHA-fSNAP-Phleomycin

    Plasmid

    #188915

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-Phleomycin
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB4283

    Plasmid

    #98702

    Purpose

    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-mTID-HA3-HIS3MX6

    Plasmid

    #227983

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-SKII-3XHA-HALO-Hygromycin

    Plasmid

    #188929

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Hygromycin
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge