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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,336 results for: G3

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Showing: 1 - 20 of 1336 results

  7. GGE261

    Plasmid
    #120783
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 3 (G3_mTurquoise)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    G3_mTurquoise
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120783-plasmid-map-sequence-id-263181

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  8. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  11. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173202-plasmid-map-sequence-id-339519

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  14. pY036_ATP1A1_G3

    Plasmid
    #86617
    Purpose
    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86617-plasmid-map-sequence-id-168852

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  19. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141273-plasmid-map-sequence-id-288100

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  20. pDONR207 SARS-CoV-2 M

    Plasmid
    #141274
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141274-plasmid-map-sequence-id-288102

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Showing: 1 - 20 of 1336 results