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pAM358
Plasmid#226138PurposeExpression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBDDepositorInsertHis6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pQE9-His-p97(wt)
Plasmid#14666DepositorInsertp97 (Vcp Mouse)
UseTagsRGS6xHisExpressionBacterialMutationSee depositor comments below.PromoterAvailable sinceApril 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330)
Plasmid#87738PurposeBacterial expression of the VSL domain of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-279PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MYMH41
Plasmid#138035PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-7GT-GFP
UseTagsExpressionBacterialMutationAAA GAG TAG AAAPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b F130W
Plasmid#1309DepositorInsertHsp104 F130W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Phe 130 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y257W
Plasmid#1310DepositorInsertHsp104 Y257W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 257 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y466W
Plasmid#1311DepositorInsertHsp104 Y466W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 466 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y507W
Plasmid#1312DepositorInsertHsp104 Y507W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 507 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b L553W
Plasmid#1313DepositorInsertHsp104 L553W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Leu 553 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y819W
Plasmid#1314DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b L892W
Plasmid#1315DepositorInsertHsp104 L892W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Leu 892 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_20aa
Plasmid#23970DepositorInsertUb-G76V-eGFP-V5HIS_20aa
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)
Plasmid#87735PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y819W
Plasmid#1307DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only
