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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,028 results for: golden gate assembly

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Showing: 301 - 320 of 1028 results

  4. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Insert
    tetA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149379-plasmid-map-sequence-id-308791

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  5. (P1)PA1lacO1

    Plasmid
    #251365
    Purpose
    IPTG-inducible Plac promoter variant with lacIq repressor and RiboJ
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    Promoter A1lacO-1
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251365-plasmid-map-sequence-id-503679

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  6. (P6)RBSS3

    Plasmid
    #251388
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251388-plasmid-map-sequence-id-503708

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  7. (P4)RBSS1

    Plasmid
    #251376
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251376-plasmid-map-sequence-id-503738

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  8. (P6)RBSS1

    Plasmid
    #251386
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251386-plasmid-map-sequence-id-503734

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  9. (P2)RBSS1

    Plasmid
    #251366
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251366-plasmid-map-sequence-id-503714

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  10. (P2)RBSS3

    Plasmid
    #251368
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251368-plasmid-map-sequence-id-503703

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  11. (P4)RBSS3

    Plasmid
    #251378
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251378-plasmid-map-sequence-id-503698

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  12. (P4)RBSS4

    Plasmid
    #251379
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251379-plasmid-map-sequence-id-503681

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  13. (P6)RBSS4

    Plasmid
    #251389
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251389-plasmid-map-sequence-id-503678

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  14. (P2)RBSS4

    Plasmid
    #251369
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251369-plasmid-map-sequence-id-503676

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  15. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

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  16. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  17. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  18. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  19. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  20. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

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Showing: 301 - 320 of 1028 results