We narrowed to 9,441 results for: pho

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EGFP-SopB(K525A)

Plasmid

#183659

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K525A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(K528A)

Plasmid

#183660

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K528A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(33-554)

Plasmid

#183661

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 33-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(33-520)

Plasmid

#183662

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 33-520

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(68-554)

Plasmid

#183663

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 68-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD(C439S)

Plasmid

#183673

Purpose

Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Mutation

C439S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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SopB(C460S)-EGFP

Plasmid

#183656

Purpose

C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

C460S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(C460S)

Plasmid

#183658

Purpose

N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

C460S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Human SHIP2

Plasmid

#124645

Purpose

Expresses HIS tagged human SHIP2 in COS-7/HEK cells

Depositor

Christophe Erneux

Article

Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

Insert

INPPL1 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Mammalian

Mutation

coding sequence starts at amino acid 18 as calcul…

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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His6-SMT3-SopB(C460S)

Plasmid

#183677

Purpose

Recombinant Salmonella Typhimurium SopB (catalytically inactive)

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)

Tags

His6-S. cerevisiae SMT3(2-101)

Expression

Bacterial

Mutation

C460S

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(172-554)

Plasmid

#183664

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 172-554

Promoter

CMV

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(211-554)

Plasmid

#183665

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 211-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-cPHx1

Plasmid

#183674

Purpose

PtdIns(3,4)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 169-329

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx2

Plasmid

#183654

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUG34-NES-EGFP-cPHx3

Plasmid

#183669

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-BTKx2

Plasmid

#183652

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-BTKx1

Plasmid

#183651

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUG36-NES-EGFP-cPHx3

Plasmid

#183668

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NES-mCherry-PH-BTKx1

Plasmid

#183653

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-N-TEV-Twin-Strep

Plasmid

#222014

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

Depositor

Chiaki Murakami

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and Twin-strep-tag

Expression

Bacterial

Promoter

lac promoter

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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