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Showing: 301 - 320 of 9015 results

  3. pGEX-N-TEV-Twin-Strep

    Plasmid
    #222014
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli
    Depositor
    Chiaki Murakami
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222014-plasmid-map-sequence-id-457136

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  4. pAcGFP-C1-DGKd2

    Plasmid
    #224897
    Purpose
    Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    224897-plasmid-map-sequence-id-456312

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  5. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Jun Yin
    Article
    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d
    Insert
    Sfp-R44
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173491-plasmid-map-sequence-id-349047

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  7. HGXPRT

    Plasmid
    #64201
    Purpose
    Expression of Plasmodium falciparum HGXPRT
    Depositor
    Vern Schramm
    Article
    Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi
    Insert
    Hypoxanthine guanine xanthine phosphoribosyltransferase
    Use
    Tags
    Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

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  12. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223721-plasmid-map-sequence-id-457432

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  13. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  16. pCapV

    Plasmid
    #183028
    Purpose
    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase CapV
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183028-plasmid-map-sequence-id-361615

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  17. pNAS1b split mCherry NEDD4 WW2 neg ctrl

    Plasmid
    #112030
    Purpose
    Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112030-plasmid-map-sequence-id-217140

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  18. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid
    #107163
    Purpose
    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme
    Depositor
    Jose Carlos Cameselle
    Article
    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.
    Insert
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107163-plasmid-map-sequence-id-204806

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  19. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  20. pCapVQ329R

    Plasmid
    #183029
    Purpose
    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase variant CapVQ329R
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    glutamine 329 changed to arginine
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183029-plasmid-map-sequence-id-361616

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Showing: 301 - 320 of 9015 results