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We narrowed to 10,228 results for: T7

Showing: 6361 - 6400 of 10228 results

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pET15b/GgVcl 1-258 A50I mutation

Plasmid

#46175

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 A50I (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-258 (VD1) A50I mutation; talin binding defic…

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TAL3322

Plasmid

#42785

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr5a2-left (nr5a2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
PCx35CT-S276A, S298ter

Plasmid

#42914

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser276 to Ala and Ser298 stop codon

Promoter

T7

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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TAL3345

Plasmid

#42808

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-prothymosin alpha a-right (ptmaa Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3067

Plasmid

#41241

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-elk1-Right (elk1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
TAL3176

Plasmid

#41350

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-solute-carrier-family-16-Left (slc16a10 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3177

Plasmid

#41351

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-solute-carrier-family-16-Right (slc16a10 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3066

Plasmid

#41240

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-elk1-Left (elk1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL2390

Plasmid

#36797

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FES-TALEN_Left (Fes Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2391

Plasmid

#36798

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FES-TALEN_Right (Fes Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2286

Plasmid

#36721

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXC13-TALEN_Left (HOXC13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2287

Plasmid

#36722

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXC13-TALEN_Right (HOXC13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
CaRQ

Plasmid

#34558

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

CaRQ (RHOA Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKV NS2B

Plasmid

#145354

Purpose

Cell-free expression vector for Zika virus NS2B protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-DMVF-181-C

Plasmid

#87611

Purpose

FKBP-tagged C-terminal Dronpa fragment for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

FKBP-DMVF181-C

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Use

Tags

His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-HIS-FLAG-AtUBQ11

Plasmid

#222720

Purpose

Expression HIS-FLAG-AtUBQ11 in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBQ11 (UBQ11 Mustard Weed)

Use

Tags

2xFLAG and 6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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PET-28a-YTH domain of YTHDF2

Plasmid

#228776

Purpose

Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

Depositor

Huiqing Zhou

Article

Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

Insert

YTH domain of YTHDF2 (YTHDF2 Human)

Use

Tags

6*His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Use

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Use

Tags

BiFC C-terminal fragment

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-tdTomato-CNA35

Plasmid

#61606

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

tdTomato and CNA35

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-mCherry-CNA35

Plasmid

#61607

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mCherry

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mCherry and CNA35

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Use

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Article

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Use

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, Markus Seeliger, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Use

Tags

Hexahistag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH-TrxT-G-L11

Plasmid

#127831

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7II-2N4Rtau

Plasmid

#177653

Purpose

Recombinant protein synthesis of human Tau

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

Tau (2N4R) (MAPT Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Use

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6361 - 6400 of 10228 results

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