We narrowed to 10,458 results for: T7

1. pBz109_CsoS2_NTD

   Plasmid

   #140842

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 N-terminal domain (NTD)
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 262-869

   Promoter

   T7

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLz47_polyPro_CsoS2_Npeptide

   Plasmid

   #140845

   Purpose

   Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 N-peptide
   

   Use

   Tags

   Hexahistidine tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBz110_CsoS2_CTD

   Plasmid

   #140844

   Purpose

   His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 C-terminal domain (CTD)
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-594

   Promoter

   T7

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHis8-4b-McDAH

   Plasmid

   #140129

   Purpose

   Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

   Depositor

   Jing-Ke Weng

   Article

   Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

   Insert

   Dechloroacutumine halogenase from Menispermum canadense
   

   Use

   Tags

   8x-His, TEV protease site (ENLYFQ)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBDuet139

   Plasmid

   #121913

   Purpose

   Charge-Introduced NpuDnaB mini-intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBΔ290 intein
   

   Use

   Tags

   GB1 and His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBDuet140

   Plasmid

   #121914

   Purpose

   Orthogonal NpuDnaB mini-intein intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBΔ290 intein
   

   Use

   Tags

   GB1 and His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNIC28-BSA4-S15

   Plasmid

   #127821

   Purpose

   Production of recombinant human ribosomal protein S15

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS15 (RPS15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNH-TrxT-G-S10

   Plasmid

   #127829

   Purpose

   Production of recombinant human ribosomal protein S10

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACYCDuet_W22HDHFR_WTTS

   Plasmid

   #91241

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W22H

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACYCDuet_F31VDHFR_WTTS

    Plasmid

    #91227

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31V

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. kank1a_pJet1.2

    Plasmid

    #121978

    Purpose

    for generating kank1a riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank1a (si:dkey-146l2.1 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MtMscL delta49

    Plasmid

    #118342

    Purpose

    M. tuberculosis MscL (delta49) in pET19b

    Depositor

    Douglas Rees

    Article

    Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

    Insert

    MscL (mscL Mycobacterium tuberculosis)
    

    Use

    Tags

    6x His Tag and Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 102 to Stop Codon

    Promoter

    T7 Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTalin2_R7R8

    Plasmid

    #111041

    Purpose

    Plasmid expresses the talin2 rod domains R7 and R8

    Depositor

    Ben Goult

    Article

    Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

    Insert

    TLN2 (Tln2 Mouse)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTalin2_R7

    Plasmid

    #111039

    Purpose

    Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

    Depositor

    Ben Goult

    Article

    Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

    Insert

    TLN2 (Tln2 Mouse)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-NmCas9-Flag D16A

    Plasmid

    #109031

    Purpose

    D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15b+R2(L171C)

    Plasmid

    #108239

    Purpose

    Expresses PdR-PCNA2(L171C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    PCNA2,L171C

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEHIShqprtR161Q

    Plasmid

    #92413

    Purpose

    hQPRTase harboring R161Q mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R161Q

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pK RagA(R37P)-RagC

    Plasmid

    #99706

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    R37P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pK RagA-RagC(L91P)

    Plasmid

    #99707

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pK RagA-RagC(K84T)

    Plasmid

    #99708

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    K84T

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pK RagA(Q66L)-RagC(Q120L)

    Plasmid

    #99702

    Purpose

    Bacterial expression of RagA(Q66L)-RagC(Q120L)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    Q120L and Q66L

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEHIAhqprtR161G

    Plasmid

    #92412

    Purpose

    hQPRTase harbouring R161G mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R161G

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEHIShqprtR102Q

    Plasmid

    #92407

    Purpose

    hQPRTase harboring R102Q mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R102Q

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEHIShqprtK171S

    Plasmid

    #92404

    Purpose

    hQPRTase harbouring K171S mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    K171S

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEHIShqprtR138Q

    Plasmid

    #92411

    Purpose

    hQPRTase harbouring R138Q mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R138Q

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEHIShqprtK139A

    Plasmid

    #92403

    Purpose

    hQPRTase harbouring K139A mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV cleacable 6xHis

    Expression

    Bacterial

    Mutation

    K139A

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEHIShqprtR102A

    Plasmid

    #92405

    Purpose

    hQPRTASE harbouring R102A mutation

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    R102A

    Promoter

    T7

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21a - TryX

    Plasmid

    #89623

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Tryparedoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPD3cC

    Plasmid

    #86972

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

    Promoter

    T7 promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p1G108CP20X

    Plasmid

    #85096

    Purpose

    Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGS-3R112C/T180C

    Plasmid

    #85095

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p1G108C-GG

    Plasmid

    #85092

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Glycine 108 to Cysteine

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmcsg7-mCRT(D311A)

    Plasmid

    #83509

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D311A

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pmcsg7-mCRT(1-351)

    Plasmid

    #83508

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    1-351 only

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

38. POLN delP_pDEST17

    Plasmid

    #64886

    Purpose

    bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

    Depositor

    Richard Wood

    Article

    Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

    Insert

    POLN (POLN Human)
    

    Use

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    with a short truncation of the C-terminal proline…

    Promoter

    T7

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSETb-pcDronpa

    Plasmid

    #78183

    Purpose

    Expresses pcDronpa in E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    pcDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Xlim-3 (lhx3)

    Plasmid

    #74139

    Purpose

    Partial cDNA clone for Xlim-3 (lhx3), named LH23-1. Cloned in BS KS+. To prepare RNA, sense: HindIII/T7; antisense: BamHI/T3.

    Depositor

    Igor Dawid

    Article

    Taira et al Dev Biol. 1993 Sep;159(1):245-56.

    Insert

    lhx3 (lhx3 Frog)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only