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CD44v-EC-His (pcDNA3)

Plasmid

#238418

Purpose

Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

CD44 containing extracellular region of CD44v (CD44 Human)

Tags

His

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11(D425A,C459S)

Plasmid

#215515

Purpose

Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-FOXC1-T2A-miRFP670

Plasmid

#182336

Purpose

Expresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box C1 (FOXC1 Human)

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233373

Purpose

EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_on-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233372

Purpose

EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_PKA_T/A-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgAAVS1_118

Plasmid

#213171

Purpose

Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_118 (AAVS1 Human)

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_FtoW

Plasmid

#224254

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_FtoW (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

F17W, F25W, F31W, F37W, F43W, F62W, F69W, F78W, F…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC#del

Plasmid

#186344

Purpose

Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Uncx [N155/9R]

Plasmid

#114545

Purpose

Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Uncx (Mus musculus) recombinant mouse monoclonal antibody (Uncx Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Uncx [N155/9R]

Plasmid

#206537

Purpose

Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Uncx (Mus musculus) recombinant Mouse monoclonal antibody (Uncx Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-SOX2-T2A-miRFP670

Plasmid

#187375

Purpose

Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

SOX2 (SOX2 Human)

Tags

T2A-miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(C)

Plasmid

#206142

Purpose

Expresses the rs27895-C allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c029

Plasmid

#175471

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

L281R, H288A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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