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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Promoter

FCP

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-Brainbow-1.0 H

Plasmid

#18720

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pCalpha EV (PKA catalytic subunit Calpha)

Plasmid

#15310

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACA (Prkaca Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pNIC-AncCAR_A

Plasmid

#168286

Purpose

Expresses AncCAR_A in E. coli from a T7 promoter

Depositor

Nicholas Harmer

Article

Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

Insert

AncCAR_A

Use

Synthetic Biology

Tags

6*His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pICH47732:FCP:NAT

Plasmid

#85984

Purpose

Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

NAT

Use

Golden gate assembly

Promoter

FCP

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Thy1-Brainbow-2.1 R

Plasmid

#18727

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pC4M-F2E-GFP-FKBP

Plasmid

#68058

Purpose

express EGFP-tagged FKBP for heterodimerization with FRB in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

EGFP

Tags

2xFKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RPA43

Plasmid

#17659

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA43 (Twistnb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSP64T Activin betaB (DM#108)

Plasmid

#15045

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

Insert

Activin Beta b (inhbb Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(504)

Plasmid

#44371

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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hSgk1_v2 Flag

Plasmid

#86040

Purpose

full length human Sgk1_i2 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

Alternate transcript v2 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v2

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-Fbw7α-3'UTR

Plasmid

#26650

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

Insert

Fbw7α

Tags

3XFLAG

Expression

Mammalian

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB

Plasmid

#15221

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTC150

Plasmid

#70014

Purpose

'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Donor + GVR

Expression

Plant

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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HaloKu70 sgRNA

Plasmid

#207583

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

GAGCAGTAGCCAACATGTCA

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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