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Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyTagsExpressionMutationbla (amp resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/UUG-nLuc-3XFLAG
Plasmid#127302PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-SIINFEKL
Plasmid#174601Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitopeDepositorInsertmembrane bound CD19, SIINFEKL (ovalbumin epitope)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C147S_pQE30
Plasmid#48631PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion E147B, C147SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateTagsExpressionMutationL22F F31SPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 T3550A/S3556A
Plasmid#199574PurposeExpresses FLAG-tagged mutated SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationT3550A and S3556APromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL001_Hec1-ms_IgG_HC
Plasmid#193618PurposeHec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.DepositorInsertAnti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
UseTagsHis6 and signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL002_Hec1-ms_IgG_LC
Plasmid#193619PurposeHec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.DepositorInsertAnti-Hec1 light chain (Hec1-ms_IgG_LC)
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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Axin2 probe
Plasmid#21277DepositorInsertAxin2 (Axin2 Mouse)
UseFor riboprobe synthesisTagsExpressionMutationcDNA bp 1774 to 2787PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-pGAS5
Plasmid#46371DepositorInsertGAS5 (GAS5 Human)
UseLuciferaseTagsluciferaseExpressionMutationpromoterPromoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9-Puro
Plasmid#135965PurposeExpresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorInserterythropoietin (epoa Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Klf4
Plasmid#19764DepositorInsertKlf4 (Klf4 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7
Plasmid#64986PurposeExpresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MGT1-d2EGFP_CMV-mCherry
Plasmid#213763PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV-HIS3
Plasmid#65333Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUsDepositorInsertHIS3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only