We narrowed to 9,520 results for: try

1. ND4 left TALE–G1333 DddAtox C

   Plasmid

   #158092

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-REDD1 48-232 (#182)

   Plasmid

   #99920

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 48-232

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI.neo-rPAM1-M314I

   Plasmid

   #145802

   Purpose

   expresses catalytically inactive rat PAM-1 in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   changed methionine 314 to isoleucine to inactivat…

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ND4 right TALE–G1333 DddAtox N

   Plasmid

   #158096

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. VGFR1_HUMAN_D0

   Plasmid

   #79743

   Purpose

   This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VGFR1
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. KK501: pMVP (L3-L2) FKBP DD + polyA

   Plasmid

   #121789

   Purpose

   pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   FKBP Degron Domain + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. NoTag MAP1LC3C

   Plasmid

   #137762

   Purpose

   Expression of LC3C without epitope tags

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   MAP1LC3C (MAP1LC3C Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-REDD1 77-232 (#426)

   Plasmid

   #99921

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 77-232

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE ENTR CMV CreERT2 R4-R3

    Plasmid

    #62173

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    CreERT2
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4_CELF1

    Plasmid

    #106098

    Purpose

    Entry vector for CELF1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    CELF1 (CELF1 Human)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. G1333 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157838

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Expression

    Mammalian

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAD/CMV/V5-DEST-Rac1-2G

    Plasmid

    #66112

    Purpose

    Second generation Rac1 FRET biosensor for adenovirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Adenoviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

    Plasmid

    #121835

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. G1397 DddAtox-N–SaKKH-Cas9(D10A)

    Plasmid

    #157839

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ269C2

    Plasmid

    #164716

    Purpose

    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible haid-cbe_v04 entry clo…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-MyosinIIC-C-18

    Plasmid

    #54194

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits