We narrowed to 31,929 results for: Eng

1. pdRBFC-B2-YN

   Plasmid

   #74721

   Purpose

   Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

   Depositor

   Aiming Wang

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   HA and YFP N-terminal half domain

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdRBFC-B2-YC

   Plasmid

   #74722

   Purpose

   Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

   Depositor

   Aiming Wang

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   FLAG and YFP C-terminal half domain

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI H2B-GFP

   Plasmid

   #92399

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330a dCas9-VP64

   Plasmid

   #92363

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcU6_1 sgRNA

   Plasmid

   #92395

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.1 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.1

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI Cas9 H2B-RFP

   Plasmid

   #92397

   Purpose

   CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcg 2.0-BFP-circ-arRNA backbone

   Plasmid

   #213965

   Purpose

   pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pminiTol2-HuC-NE2h

    Plasmid

    #208689

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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