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We narrowed to 26,294 results for: STI

Showing: 3221 - 3240 of 26294 results

  1. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp

    Plasmid
    #218457
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Thr1273Asp substitution
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218457-plasmid-map-sequence-id-436203

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  6. HA tagged SphI ELL2 M133, 138, 186ILeu

    Plasmid
    #127277
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Met 133, 138, 186 to Ileu; HA inserted at SphI af…
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127277-plasmid-map-sequence-id-250039

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  9. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  12. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-432916

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  13. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 (Tet1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102421-plasmid-map-sequence-id-198790

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  14. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  16. CROPseq-MPSV-eBFP2

    Plasmid
    #220472
    Purpose
    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
    Depositor
    Christian Krebs
    Article
    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    220472-plasmid-map-sequence-id-437895

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  17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

    Plasmid
    #208958
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208958-plasmid-map-sequence-id-415784

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  19. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid
    #209060
    Purpose
    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.
    Depositor
    Matthew Oser
    Article
    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.
    Insert
    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    Use
    Gateway vector to be used for lr reaction
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209060-plasmid-map-sequence-id-412264

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  20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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Showing: 3221 - 3240 of 26294 results