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Plasmid#69279PurposeGolden Gate entry vector;7th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only
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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b
Plasmid#184293PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1bDepositorInsertssgRNA-Adra1b
sgRNA-Adra1b
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD296.7
Plasmid#113320PurposedCas9, MCP-TetD, J107 scRNA.b1DepositorInsertdCas9, MCP-TetD, J107 scRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS254
Plasmid#73721PurposeSapTrap Halotag + Cbr-unc-119 donor plasmidDepositorInsertHalotag + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS297
Plasmid#73737PurposeSapTrap STOP codon + let-858 UTR N-terminal connector donor plasmidDepositorInsertSTOP codon + let-858 3'-UTR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJF121
Plasmid#113325PurposepTet-W108 scRNA.b2DepositorInsertW108 scRNA_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK504
Plasmid#155381PurposedCas12a crRNAsDepositorInsertdCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLS269
Plasmid#73727PurposeSapTrap SL2::SV40-NLS C-terminal connector donor plasmidDepositorInsertSL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
iC30 PB-Zim3-dCas9-P2A-BFP_hygro
Plasmid#236743PurposeConstitutive expression of Zim3-dCas9InsertsCAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
EF1a-hygro-WPRE
UseTagsExpressionMammalianMutationPromoterCAG and EF1aAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE mTFP1-actin
Plasmid#229970Purposeencodes a mTFP1-actin fusion construct under the control of a dox inducible promoterDepositorInsertmTFP1-actin
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE H2B iRFP720
Plasmid#229968Purposeencodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoterDepositorInsertH2B iRFP720
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB CMV
Plasmid#229953PurposebigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE mito-mCherry
Plasmid#229966Purposeencodes a mCherry targeted to mitochondria under the control of a dox inducible promoterDepositorInsertmito-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB EF1alpha
Plasmid#229954PurposebigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE GTS-mTagBFP
Plasmid#229969Purposeencodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoterDepositorInsertGTS-mTagBFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE EYFP-tubulin
Plasmid#229967Purposeencodes a YFP-tubulin fusion contruct under the control of a dox inducible promoterDepositorInsertEYFP-tubulin
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psLIB TRE
Plasmid#229955PurposebigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204729PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204727PurposeFrt-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP
Plasmid#204724PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh8-2300
Plasmid#170986PurposeEncodes sgRNA target 2300 and Hygromycin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp
Plasmid#184292PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting GrpDepositorInsertsgRNA-Grp
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1
Plasmid#184291PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1DepositorInsertsgRNA-Oprl1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3
Plasmid#184290PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3DepositorInsertsgRNA-Rxfp3
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184295PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC5 cloning template vector
Plasmid#183424PurposeFlpOFF knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKEW395_AcrIIA14_truncated
Plasmid#138284PurposeExpression of truncated AcrIIA14 proteinDepositorInserttruncated AcrIIA14
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti
Plasmid#138312PurposeLenti expression of AcrIIA14, C-terminusDepositorInsertAcrIIA14, C terminus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW353_AcrIIA13_truncated
Plasmid#138282PurposeExpression of truncated AcrIIA13 proteinDepositorInserttruncated AcrIIA13
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW398_AcrIIA15_truncated
Plasmid#138286PurposeExpression of truncated AcrIIA15 proteinDepositorInsertAcrIIA15
UseCRISPRTagsSFGFPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118160PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNR2F1.1.0-gDNA
Plasmid#112484PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1DepositorInsertNR2F1 (NR2F1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMAD3.1.0-gDNA
Plasmid#112407PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3DepositorInsertSMAD3 (SMAD3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS292
Plasmid#73725PurposeSapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmidDepositorInsert2xNLS-mCherry + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoCDA1
Plasmid#195515PurposeUbiquitous expression of dCBEevoCDA1 in DrosophilaDepositorInsertdCBE-evoCDA1
UseCRISPRTagsExpressionInsectMutationPromoteract5CAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEgRNA
Plasmid#172716PurposeDelivery of guide RNA for prime editingDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA
Plasmid#179211PurposeGolden Gate entry vector; 2nd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA
Plasmid#114005Purposeexpress sgRNA. ColE1, KanDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnsgRNA
Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd2
Plasmid#159918PurposeMutagenesis of Drd2DepositorInsertDrd2 (Drd2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1
Plasmid#159905PurposeMutagenesis of Slc32a1DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDrd1
Plasmid#159917PurposeMutagenesis of Drd1DepositorInsertDrd1 (Drd1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT32
Plasmid#204297PurposepJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B
Plasmid#179216PurposeGolden Gate entry vector; 4th gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAtU3Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK33/Cas9
Plasmid#133781PurposeExpresses drosophila-optimized Cas9DepositorInsertDrosophila-optimized Cas9
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
rGluA2 (px330:2-guideCas9)
Plasmid#169439PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only