We narrowed to 12,964 results for: BASE

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Pink Flamindo

Plasmid

#102356

Purpose

biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

Insert

Pink Flamindo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-dTAG

Plasmid

#207683

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-dTAG

Plasmid

#207705

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUGI-NLS

Plasmid

#101091

Purpose

Expresses UGI in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

UGI

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1

Plasmid

#237695

Purpose

Inducible lentiviral expression of RNaseH1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBIS

Plasmid

#35150

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Promoter

LacUV5,

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

Plasmid

#185914

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCT-tRNA

Plasmid

#133813

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter

Use

CRISPR

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Zeo

Plasmid

#227255

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-synORP1

Plasmid

#191759

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD

Plasmid

#154938

Purpose

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-(-2)AP-Ub

Plasmid

#232582

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

Plasmid

#185919

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mCherry-3XNLS-WPRE

Plasmid

#159951

Purpose

nucleus marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-3XNLS

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-ORP1

Plasmid

#191677

Purpose

Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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