We narrowed to 22,374 results for: ESC;

1. pCHMP4B-NS3-Green

   Plasmid

   #223528

   Purpose

   Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

   Depositor

   Winfried Weissenhorn

   Article

   Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

   Insert

   CHMP4B (CHMP4B Human)
   

   Tags

   NS3 Hepatitis C protease and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC364 - pAAV CaMKIIa iRFP

   Plasmid

   #47903

   Purpose

   An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF11-wNIR-GECO2-T2A-HO1

   Plasmid

   #159606

   Purpose

   Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

   Depositor

   Robert Campbell

   Article

   Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

   Insert

   wNIR-GECO2-T2A-HO1
   

   Expression

   Worm

   Promoter

   tag-168

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HA-CDK2

   Plasmid

   #172613

   Purpose

   Expresses HA-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159676

   Purpose

   Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Chicken Mermaid S188

   Plasmid

   #53617

   Purpose

   Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Chicken Mermaid S188
   

   Expression

   Mammalian

   Mutation

   Gg-VSP contains an R153Q mutation and an amino ac…

   Promoter

   CMV

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC588 - pAAV c-fos Nuc-iRFP

   Plasmid

   #59132

   Purpose

   An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Nuclear-localized Infrared Fluorescent Protein
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   mFos

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UBC-S3-ExRaiAKAR

   Plasmid

   #160724

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S3-ExRaiAKAR
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UBC-S4-ExRaiCKAR

   Plasmid

   #160725

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S4-ExRaiCKAR
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL

    Plasmid

    #119952

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159677

    Purpose

    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. M77

    Plasmid

    #242629

    Purpose

    MYC knockdown + rescue

    Depositor

    Alain Nepveu

    Article

    Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    V5-MYCMutMBIIIa, MutMBIV (MYC Human)
    

    Use

    RNAi

    Tags

    V5

    Mutation

    I189A,V193A,V194A,F195S,L199A,A310E,A311D,P312G,P…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCpep-E1GFP

    Plasmid

    #233884

    Purpose

    Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

    Depositor

    Francesco Cardarelli

    Article

    De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

    Insert

    Proinsulin-E1GFP (Ins2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Inserted E1GFP into C-peptide of mouse Ins2 codin…

    Promoter

    CMVF

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA528 CHMP5 159-222

    Plasmid

    #108259

    Purpose

    Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP5 residues 148-219 (CHMP5 Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCHMP4B-NS3-Blue

    Plasmid

    #223534

    Purpose

    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP4B (CHMP4B Human)
    

    Tags

    NS3 Hepatitis C protease and mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCHMP4B-mut-NS3-Green

    Plasmid

    #223531

    Purpose

    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP4B (CHMP4B Human)
    

    Tags

    NS3 Hepatitis C protease and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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