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Plasmid#212724PurposeBacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET24a-NUbo-MCS-His6
Plasmid#212723PurposeBacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-95~236
Plasmid#208634Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236DepositorInserthuman RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 95~236, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.1
Plasmid#191928PurposeEncodes a GFP design, sf:em-blue-shift.1DepositorInsertsf:em-blue-shift.1
UseTagsHis TagExpressionBacterialMutationT65S Q69P S72A T108V Y145M T167V V224IPromoterT7Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24-aCatenin(82-906)-His6
Plasmid#203332PurposeBacterial expression of wild-type alpha-cateninDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags6xHisExpressionBacterialMutationaa 82-906PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED 4CA-FLAG
Plasmid#199681PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-FlagDepositorInsertLeucine zipper LYCHOS LED 4CA (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationC595.604.629.638 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED Y551A-FLAG
Plasmid#199680PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-FlagDepositorInsertLeucine zipper LYCHOS LED Y551A (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationY551 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only