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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAG66

Plasmid

#226751

Purpose

Expresses exogenous GFP

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

GFP

Use

Lentiviral

Tags

sfGFP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrH

Plasmid

#225084

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Tags

His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrI

Plasmid

#225085

Purpose

Expresses anthrone-oxygenase like enzyme ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrI

Tags

His6 and His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV100

Plasmid

#215306

Purpose

Expresses sfGFP under induction with the 3OC6-HSL Marionette Cassette (LuxR + PLuxB-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PLuxB

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV110

Plasmid

#215340

Purpose

Expresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PAcu

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV107

Plasmid

#215337

Purpose

Expresses sfGFP under induction with the DHBA Marionette Cassette (PcaUAM + P3B5B-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

P3B5B

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV105

Plasmid

#215311

Purpose

Expresses sfGFP under induction with the choline Marionette Cassette (BetIAM + PBetI-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PBetI

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGM209

Plasmid

#220174

Purpose

Expresses exogenous wild-type TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV101

Plasmid

#215307

Purpose

Expresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PVanCC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PgpdA

Plasmid

#213466

Purpose

Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of the glyceraldehyde-3-phosphate dehydrogenase

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV111

Plasmid

#215341

Purpose

Expresses sfGFP under induction with the Erythromycin Marionette Cassette (MphRAM + EryR + PMph-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PMph

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV106

Plasmid

#215312

Purpose

Expresses sfGFP under induction with the naringenin Marionette Cassette (TtgRAM + PTtG-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PTtg

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_41BBICD

Plasmid

#197097

Purpose

This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of 4-1BB.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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