We narrowed to 5,828 results for: pcas

1. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

   Plasmid

   #217803

   Purpose

   Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

   Plasmid

   #217796

   Purpose

   Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

   Plasmid

   #217797

   Purpose

   Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC-sleeping beauty

   Plasmid

   #65487

   Purpose

   HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

   Depositor

   Guillaume Filion

   Insert

   sleeping beauty transposase
   

   Expression

   Insect

   Promoter

   HSP

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2/2

   Plasmid

   #232199

   Purpose

   AAV packaging plasmid expressing AAV2 Rep and capsid proteins

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Rep2/Cap2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV2/5

   Plasmid

   #232922

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Rep2/Cap5
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 17, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV2/11

   Plasmid

   #240486

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   AAV2 Rep and AAV11 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV2/6

   Plasmid

   #240485

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   AAV2 Rep and AAV6 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid

    #136272

    Purpose

    Expresses PE2 and ST2-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2, pegRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9_P2A_T4pol

    Plasmid

    #190146

    Purpose

    Expresses SpCas9 and T4pol

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_T4pol
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9_P2A_POLB

    Plasmid

    #190145

    Purpose

    Expresses SpCas9 and POLB

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mRFP

    Plasmid

    #28311

    Depositor

    Joseph Loturco

    Article

    Manent et al Nat Med. 2009 Jan . 15(1):84-90.

    Insert

    pCAGGS-mRFP
    

    Expression

    Mammalian

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pJH1

    Plasmid

    #154204

    Purpose

    encodes CRISPR-Cas9 system derived from plasmid pCas9

    Depositor

    Russell Poulter

    Article

    Ho et al J Med Microbiol. 2020 Mar;69(3):478-486.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid

    #242651

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAK005

    Plasmid

    #233642

    Purpose

    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    eSpCas9(1.1) with c-terminal VitelloTagB
    

    Tags

    6-His

    Expression

    Mammalian

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK004

    Plasmid

    #233641

    Purpose

    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    eSpCas9(1.1) with c-terminal VitelloTagA
    

    Tags

    6-His

    Expression

    Bacterial

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.3TOPO-T7-hCas9

    Plasmid

    #161876

    Purpose

    In vitro transcription of SpCas9

    Depositor

    Stephane Pelletier

    Article

    Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

    Insert

    human codon optimized SpCas9
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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