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pICH45195

Plasmid

#50275

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFM112

Plasmid

#40158

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1C:FLAG (par-1 Nematode)

Tags

GST and modified Flag

Expression

Bacterial

Mutation

Contains aa 966-1192

Promoter

tac

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pRLCon850-1207

Plasmid

#31447

Depositor

Gerhart Ryffel

Publication

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon1-449

Plasmid

#31452

Depositor

Gerhart Ryffel

Publication

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon1-249

Plasmid

#31453

Depositor

Gerhart Ryffel

Publication

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon1-249_SNP

Plasmid

#31454

Depositor

Gerhart Ryffel

Publication

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

SNP rs11574744 in miR-34a binding site

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab8b_3

Plasmid

#26709

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b_3 RNAi.

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRab8a_2

Plasmid

#26707

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8a RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8a RNAi.

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLLNeo-Oct3

Plasmid

#21618

Depositor

David Garbers

Publication

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pFB2A

Plasmid

#195283

Purpose

Bicistronic DTR and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Hygro (HBEGF Human, Synthetic)

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

Plasmid

#114086

Purpose

CAG promoter expression plasmid for human codon optimized LbBE1.4

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)

Tags

2x SV40 NLS and SV40 NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3xFLAG-LRRK2

Plasmid

#244986

Purpose

Express LRRK2 in mammalian cells with 3xFLAG tag

Depositor

Luis Bonet-Ponce

Publication

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

LRRK2 (LRRK2 Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

CMV

Available since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-GRAB_PGE2-1.0

Plasmid

#251355

Purpose

Express the PGE2 sensor GRAB_PGE2-1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wang et al Neuron. 2026 Jun 3;114(11):1922-1934.e5.

Insert

PGE2 sensor GRAB_PGE2-1.0

Use

AAV

Expression

Mammalian

Promoter

EF1α

Available since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NPY pHLeon

Plasmid

#235176

Purpose

Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

Depositor

Kate White

Publication

Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

Insert

NPY-pHLeon (NPY Human)

Expression

Mammalian

Promoter

CMV

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pNX24-RFP-MS2SL

Plasmid

#227819

Purpose

Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

Depositor

Jeffrey Gerst

Publication

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Inserts

3xHA-RFP-24xMS2SL
Neo resistance

Expression

Mammalian

Promoter

CMV and SV40

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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