We narrowed to 7,278 results for: 11

1. PatoL-OG241

   Plasmid

   #72841

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. DT12A

   Plasmid

   #80416

   Purpose

   Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST39-Hrs-STAM1 core

   Plasmid

   #21497

   Depositor

   James Hurley

   Article

   Ren et al Structure. 2009 Mar 11. 17(3):406-16.

   Insert

   pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

4. MTK2_007

   Plasmid

   #123702

   Purpose

   Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pEF1a
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTW072M

   Plasmid

   #115960

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl2 (nsP2 Q739L)
   

   Use

   Synthetic Biology

   Mutation

   nsP2 Q739L

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv1 sgTollip

   Plasmid

   #196546

   Purpose

   Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3_V5-APEX2-Sec61B

   Plasmid

   #83412

   Purpose

   Expressed N-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-Sec61B (SEC61B Human, Synthetic)
   

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3_HMOX1-V5-APEX2

   Plasmid

   #85823

   Purpose

   Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
   

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-T-GECO1

   Plasmid

   #191616

   Purpose

   Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

   Depositor

   Robert Campbell

   Article

   Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

   Insert

   T-GECO1
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FL tension sensor module

    Plasmid

    #101170

    Purpose

    Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

    Depositor

    Carsten Grashoff

    Article

    Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

    Insert

    YPet(short)-FL-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK4_003

    Plasmid

    #123812

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p55-H2B-Dendra2

    Plasmid

    #80609

    Purpose

    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    H2B-Dendra2 (H2BC21 Human, Synthetic)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CFP-CD82

    Plasmid

    #1818

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    CD82 (CD82 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. plvx-mTSPAN9-mCherry

    Plasmid

    #210337

    Purpose

    Overexpression of mTSPAN9

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN9 (Tspan9 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag MKK3b(Glu)

    Plasmid

    #50449

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

17. His-Keap1-Tev-Halo

    Plasmid

    #87545

    Purpose

    expresses C-terminal Halo-tagged human Keap1 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    Halo and His-Tag

    Expression

    Mammalian

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQCXIP TBRII

    Plasmid

    #19147

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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