We narrowed to 17,105 results for: STO
-
Plasmid#111149PurposeExpresses arginylated human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationN-terminal arginylationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ACE2 receptor 19-615
Plasmid#167012PurposeGateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2DepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationAmino acids 19-615, No stop codonPromoterAvailable sinceMarch 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-nAChr-Alpha6-YFP
Plasmid#50483Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsYFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the YFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
RPK2 X042_pECIA2
Plasmid#115135PurposeBait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.DepositorInsertAT3G02130 (RPK2 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-IRES_ECFP
Plasmid#48354PurposeContributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.DepositorInsertIRES_ECFP
UseGateway entry vectorTagsExpressionMutationECFP translation is mediated by an IRES. Contains…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rb379-928/C706F-pGex2TK
Plasmid#21750DepositorInsertRb (RB1 Human)
UseTagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928 of wt Rb …PromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD
Plasmid#225872PurposeLentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationamino acids 1-44 from H3.3 K36MPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN
Plasmid#172435PurposeLentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKLDepositorInsertsCre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EGFP.T2A.NCLX.3'UTR
Plasmid#181872PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)DepositorInsertsUseAAV and AdenoviralTagsHA, T2A, and mycExpressionMammalianMutationincludes a large portion of the Slc8b1 3'UTR…Promotersynthetic hybrid CAG promoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-Cavin4b_PRDmt
Plasmid#176017PurposeMultisite gateway middle entry clone containing zebrafish Cavin4b_PRDmt with no stop codon. Parton lab clone JLY.DepositorInsertCavin4b (PRDmt) (cavin4b Zebrafish)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK-Ldb3-mKate2
Plasmid#128766PurposeFluorescent mKate reporter for Ldb3DepositorInsertLdb3 promoter (Ldb3 Mouse)
UseTagsExpressionMammalianMutationPromoterLdb3Available sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rosa26-LSL-PE2 Targeting Vector
Plasmid#196038PurposeThe targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.DepositorInsertCas9-MMLV-RT
UseMouse TargetingTagsExpressionMammalianMutationPromoterCAGGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0-6H-TEV-KRAS G12D
Plasmid#159439PurposeExpresses KRAS G12D in E.coliDepositorInsertKRAS G12D (KRAS Human)
UseTags6xHISExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry
Plasmid#181853PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
KRAS-A146T
Plasmid#154426PurposeA146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 GST-hUSP27X K181E
Plasmid#225714PurposeBacterial expression of USP27X (K181E) with GST tagDepositorInsertUSP27X K181E (USP27X Human)
UseTagsGSTExpressionBacterialMutationK181EPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro 3XFLAG-hATXN7L3
Plasmid#225725PurposeTransfection of ATXN7L3 with 3XFLAG tag in mammalian cellsDepositorInsertATXN7L3 (ATXN7L3 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro HA-hUSP27X S404N
Plasmid#225724PurposeTransfection of USP27X (S404N) with HA tag in mammalian cellsDepositorInsertUSP27X S404N (USP27X Human)
UseTagsHAExpressionMammalianMutationS404NPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only