We narrowed to 4,353 results for: erf

1. pdCas9-J23109

   Plasmid

   #113151

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT119b

   Plasmid

   #50551

   Purpose

   Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Inserts

   CcaS
   CcaR
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZL Hygro-NMYC TRF2 deltaB deltaM

   Plasmid

   #16355

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF2 (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

   Promoter

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pWS5399-CRISPRai-ZeoR

   Plasmid

   #182838

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWS5321-CRISPRai-Markerless

   Plasmid

   #182828

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - markerless

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEutRwc

   Plasmid

   #216334

   Purpose

   An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

   Depositor

   Mark Styczynski

   Article

   McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

   Inserts

   EutR
   Superfolder green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAbaMCi-BsaI_pJMP2776

   Plasmid

   #208890

   Purpose

   Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM704

   Plasmid

   #68891

   Purpose

   IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSX2720

   Plasmid

   #200764

   Purpose

   Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

   Depositor

   Suhong Xu

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   scFv-sfGFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   col-19

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMP71-IFN-B

    Plasmid

    #174597

    Purpose

    Expresses secreted murine interferon beta

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    secreted IFN-beta
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWS5398-CRISPRai-HygR

    Plasmid

    #182837

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS5390-CRISPRai-URA3

    Plasmid

    #182829

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LMAPCW

    Plasmid

    #205655

    Purpose

    shRNAmir backbone for RNA interference, with mCherry reporter

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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