We narrowed to 3,423 results for: mpo

1. pSFG-TFII-I-GFP Y248&249F

   Plasmid

   #22196

   Depositor

   Ananda Roy

   Article

   Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Tyrosines 248 and 249 to Phenylalanines. …

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

   Plasmid

   #233388

   Purpose

   PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   IL2Rb(ecto+TM)-ADAR2dd
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

   Plasmid

   #233385

   Purpose

   PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   VEGFR1(ecto+TM)-ADAR2dd
   

   Use

   Tags

   FLAG, HA

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

   Plasmid

   #231345

   Purpose

   Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7/T3-BC(p1-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 - circMAN2A1

   Plasmid

   #198837

   Purpose

   This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

   Depositor

   Stefan Stamm

   Article

   Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

   Insert

   circular mannosidase alpha class 2A member 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   circular RNA composed by 2 exons

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2602

   Plasmid

   #142964

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU6F1 (POU6F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF3522

   Plasmid

   #144998

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU6F1 (POU6F1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro Cas13d-eGFP U6 RUNX1 g1

   Plasmid

   #155185

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   Cas13d RUNX1 gRNA1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121803

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid

    #121811

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121815

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121820

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JH204: pMVP (R4-R3) human PDX1 open

    Plasmid

    #121745

    Purpose

    pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Human PDX1 (open) (PDX1 Human)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid

    #121748

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag-IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. N-Flag-IPO9_Lenti SFFV Puro WPRE

    Plasmid

    #217916

    Purpose

    Express IPO9 in mammalian cells

    Depositor

    Benjamin Cravatt

    Article

    Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

    Insert

    importin 9 (IPO9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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