We narrowed to 11,993 results for: pcdna

1. pcDNA5FRTTO-NHASt-codoptUL50

   Plasmid

   #135101

   Purpose

   Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-mTRAF3ip3-CFP

   Plasmid

   #125181

   Purpose

   Expresses mouse TRAF3IP3 in mammalian cells

   Depositor

   Shao-Cong Sun

   Insert

   TRAF3ip3 (Traf3ip3 Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   K87R, M283I, S359P, A506G- please see depositor c…

   Promoter

   CMV

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Nluc(W12stop)

   Plasmid

   #180502

   Purpose

   Mammalian expression of Nluc(W12stop)

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Nluc(W12stop)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W12(UGG) to amber stop codon(UAG)

   Promoter

   CMV

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Flag-EcoCas6

   Plasmid

   #106274

   Purpose

   Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasE
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-FH-Nsp7

   Plasmid

   #157689

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCDNA3.1-nAChr-beta3

   Plasmid

   #48811

   Purpose

   This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

   Depositor

   Jerry Stitzel, Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. a4 ecto pcDNA3.1 hygro(-)

   Plasmid

   #113406

   Purpose

   expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

   Insert

   human alpha 4 ectodomain
   

   Use

   Tags

   Strep II tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO DNAJB2b

   Plasmid

   #19470

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB2b (DNAJB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5-FRT-TO-Nsp15-HF

    Plasmid

    #157722

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA-GATA2-DC+Nzf

    Plasmid

    #1293

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-HA-ERK2 L198A

    Plasmid

    #8975

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A

    Promoter

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-sACE2v2.2-IgG1

    Plasmid

    #149667

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. XE212 RGS9-pcDNA3.1

    Plasmid

    #16676

    Depositor

    Randall Moon

    Insert

    RGS9 (Rgs9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-FLAG-Lin28BdelN70

    Plasmid

    #51374

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 70 amino acids deletion

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-Q842gp120-8his

    Plasmid

    #100932

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-hDDB2-T338M-V5

    Plasmid

    #167470

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T338M

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sACE2v4-Fc(IgG1)

    Plasmid

    #145167

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-CCND1(T286A)

    Plasmid

    #172631

    Purpose

    Expresses untagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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