We narrowed to 6,951 results for: ust

1. cpVenus-Gγ2

   Plasmid

   #69626

   Purpose

   The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   cp173Venus-Ggamma2 (GNG2 Synthetic)
   

   Use

   Tags

   ciruclar permutated Venus (cp173V)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EGFR-L858R-T790M-dual-epegRNA

   Plasmid

   #214100

   Purpose

   Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R epegRNA/EGFR T790M epegRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB-GST-IRP2

   Plasmid

   #226621

   Purpose

   Expresses human IRP2 in insect cells

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Insert

   IRP2 (IREB2 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Basigin-isoform2-CD4d3+4-bio

   Plasmid

   #73129

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Basigin isoform 2 (BSG Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHES839

   Plasmid

   #87941

   Purpose

   Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 (crippled)

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-DIO-BiPOLES-mCerulean

   Plasmid

   #154949

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXL002-ishRNA-beta-catenin-1

   Plasmid

   #36297

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid

    #140199

    Purpose

    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

    Plasmid

    #160046

    Purpose

    For expressing the full paradoxical circuit

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Inserts

    osTIR1
    iaaH
    iCasp9
    BlastR
    

    Use

    Synthetic Biology

    Tags

    mAID, mCherry, and mGFPmut3

    Expression

    Mammalian

    Mutation

    Promoter

    no promoter, T2A is used and pEF1s

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid

    #140197

    Purpose

    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNase deactivated

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-DIO-BiPOLES-mCerulean

    Plasmid

    #154950

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid

    #183765

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    ChR2-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXL003-ishRNA-beta-catenin-2

    Plasmid

    #36299

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-2 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

16. mDlx-BiPOLES-mCerulean

    Plasmid

    #154946

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal Dlx

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a driving selection cassette

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Foxf1 pcDNA HisC

    Plasmid

    #122119

    Purpose

    Expression of Foxf1 in mammalian cells

    Depositor

    Paul Herring

    Article

    Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

    Insert

    Foxf1 (Foxf1 Mouse)
    

    Use

    Tags

    6xHis and Express

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX0393

    Plasmid

    #205984

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGST-DHFR Mm

    Plasmid

    #83073

    Purpose

    Expresses the full length DHFR Mm in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    2x DHFR (Dhfr Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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