We narrowed to 8,417 results for: Ott

1. pCFJ910

   Plasmid

   #44481

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, NeoR, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEmCherry2

   Plasmid

   #118413

   Purpose

   Red fluorescent reporter for assessing CRISPR gRNA activity

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT-GEM(2)

   Plasmid

   #62894

   Purpose

   Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-puro-2A-TEVp

   Plasmid

   #99610

   Purpose

   Lentivirus expressing puromycin resistance gene and TEV protease

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   Puromycin resistance (PAC gene)
   TEV protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmScarlet_NES_C1

   Plasmid

   #85060

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmScarlet-I_peroxisome_C1

   Plasmid

   #85065

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FSW-HRP-V5-SLITRK3

   Plasmid

   #82539

   Purpose

   Lentiviral vector for expressing HRP-SLITRK3 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   SLITRK3 (SLITRK3 Human)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FSW-HRP-V5-NLGN2

   Plasmid

   #82545

   Purpose

   Overexpression vector for V5-NLGN2 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   NLGN2 (Nlgn2 Rat)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-mCherry_SNAP_SNAPV_PACT_Active

   Plasmid

   #173072

   Purpose

   Active SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Use

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VinculinTS-A50I/I997A

    Plasmid

    #111829

    Purpose

    Vinculin tension sensor with point mutations affecting ability to bind talin and actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed vinculin alanine 50 to isoleucine and iso…

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ909

    Plasmid

    #44480

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, MCS
    

    Use

    Multiple cloning site vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT-GEM(1)

    Plasmid

    #62893

    Purpose

    Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR1A-3XFlag-WT-MEFV

    Plasmid

    #167018

    Purpose

    Gateway entry plasmid with 3xflag-pyrin (Wild-type)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Ii-OVA.II

    Plasmid

    #64597

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 315-353 (class II epitopes)

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB-Flag-MyoD-MyoD

    Plasmid

    #25992

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD-MyoD homodimer (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pAcGFP1-N1-MAHS

    Plasmid

    #90034

    Purpose

    To express GFP-tagged MAHS in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    MAHS
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS_Transposase

    Plasmid

    #109768

    Purpose

    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Tol2 Transposase
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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