We narrowed to 8,101 results for: gnal

1. pcDNA3 Flag Rab1a N124I

   Plasmid

   #46778

   Purpose

   expression of N124I Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a (Rab1a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N124I cannot stably bind guanine nucleotide

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. CLPIT Cry2PHR-mCh-Rac1

   Plasmid

   #42955

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-Off promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR-siKlf5

   Plasmid

   #41740

   Depositor

   Jonathan Katz

   Article

   Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. secCitrine

   Plasmid

   #181964

   Purpose

   Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   signal peptide sequence from basic chitinase
   

   Use

   Tags

   Citrine YFP

   Expression

   Plant

   Mutation

   Promoter

   1.5 kb MUM4 promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ad-HisFlag-USP19CYT

   Plasmid

   #155245

   Purpose

   for generating adenoviruses expressing the cytoplasmic isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

   Insert

   USP19 (Cytoplasmic isoform) (Usp19 Rat)
   

   Use

   Adenoviral

   Tags

   6XHis-3XFlag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP-Gamma 5

   Plasmid

   #36044

   Depositor

   Narasimhan Gautam

   Article

   Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

   Insert

   G protein subunit Gamma 5 (Gng5 Rat)
   

   Use

   Tags

   His and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLL7.0: Venus-oLID-Mito (From ActA)

   Plasmid

   #60414

   Purpose

   Mammalian Expression of Venus-oLID-Mito

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-oLID-Mito
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIC389

   Plasmid

   #37387

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   LGN (GPSM2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-364 and 524

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIBA2-SpyTag-MBP

   Plasmid

   #107422

   Purpose

   Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   Maltose-binding protein (MalE)
   

   Use

   Tags

   OmpA signal peptide, SpyTag, and StrepII

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcFLAG-Grap2-WT

    Plasmid

    #74511

    Purpose

    Overexpression of Grap2 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pECFP-Cdc42-Y40C

    Plasmid

    #105295

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mSmo-NQ4-V5

    Plasmid

    #126416

    Purpose

    Localization, Expression of Smoothened NQ4 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ4

    Promoter

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS U6 WIP RNAi

    Plasmid

    #11774

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP RNAi (WIPF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. NmMetQ N238A

    Plasmid

    #129648

    Purpose

    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

    Depositor

    Douglas Rees

    Article

    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

    Insert

    metQ Gene
    

    Use

    Tags

    10x Histidine Tag, Enterokinase, and pelB signal …

    Expression

    Bacterial

    Mutation

    Changed Asparagine 238 to Alanine

    Promoter

    T7 Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-MCPIP1(1-453)

    Plasmid

    #90106

    Purpose

    Mammalian expression of Flag-MCPIP1(1-453)

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag-Sesn1b

    Plasmid

    #61867

    Purpose

    Transient overexpression of Flag-Sesn1b (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn1 (Sesn1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGL2-Skp2 promoter-luciferase

    Plasmid

    #81119

    Purpose

    Reporter activity of SKP2 gene promoter

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (Skp2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    contains the region from 1_kb upstream of transcr…

    Promoter

    SKP2

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1/3xmyc_NLRP11-NACHT

    Plasmid

    #131199

    Purpose

    mammalian expression

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    NACHT of NLRP11 (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains only the NACHT domain (amino acids 125-5…

    Promoter

    pCMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 AT1AR-324delta

    Plasmid

    #45635

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-324delta (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    added stop codon at 324 aa

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

20. PhyB-mCherry-MYO1

    Plasmid

    #51570

    Purpose

    Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    MYO1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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