We narrowed to 6,927 results for: 11

1. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pINSPECT:NEAT1-v2_SigP:NLuc

   Plasmid

   #197268

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-v2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106190

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   CAG

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106179

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   hSynapsin

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

   Plasmid

   #114091

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (E174R/S542R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R and S542R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1α1.1-Chronos-GFP

   Plasmid

   #122098

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. shCCL20

   Plasmid

   #65096

   Purpose

   retroviral expression of CCL20 shRNA

   Depositor

   Pau Sancho-Bru

   Article

   Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

   Insert

   Ccl20 shRNA (Ccl20 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106185

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   hSynapsin

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106191

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106184

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    hSynapsin

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmeIF4E

    Plasmid

    #79258

    Purpose

    Expresses EGFP-tagged DmeIF4E in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    eIF4E (eIF4E1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MBP-rPKL WT

    Plasmid

    #107156

    Purpose

    MBP-rPKL WT for production and purification of rat PKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-hPKL S113D

    Plasmid

    #107161

    Purpose

    For mammalian expression of Flag-hPKL S113D

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    Flag-hPKL S113D (PKLR Human)
    

    Use

    Retroviral

    Tags

    N-term Flag

    Expression

    Mammalian

    Mutation

    Serine 113 mutated to aspartate

    Promoter

    CMV

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-hPKL S113A

    Plasmid

    #107160

    Purpose

    For mammalian expression of Flag-hPKL S113A

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    Flag-hPKL S113A (PKLR Human)
    

    Use

    Retroviral

    Tags

    N-term Flag

    Expression

    Mammalian

    Mutation

    Serine 113 mutated to alanine

    Promoter

    CMV

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.GFAP.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106196

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    GFAP

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psiCHECK2-AURKB 3'UTR

    Plasmid

    #29475

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    AURKB 3"UTR (AURKB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAAV.GFAP.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106197

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    GFAP

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMiR-CIP2A-miR-375-mutA-E

    Plasmid

    #53754

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A-E (refer to ci…

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pYO1025

    Plasmid

    #235705

    Purpose

    Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Inserts

    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    3xTEV-ZZ

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CAG-VEGF120-T2A-mCherry

    Plasmid

    #229134

    Purpose

    Retrovirus driving overexpression of VEGF120 plus mCherry reporter

    Depositor

    Elizabeth Kirby

    Article

    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

    Inserts

    VEGF120 (Vegfa Mouse)
    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CRaf-IRES-pErk reporter

    Plasmid

    #226170

    Purpose

    Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)
    

    Use

    Tags

    3xFlag-tdMCP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LMPd Ametrine Got1 shRNA#1

    Plasmid

    #220592

    Purpose

    Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

    Insert

    Got1 shRNA (Got1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE-RnCENP-O-11NS-GFP

    Plasmid

    #205181

    Purpose

    Expresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo, Cenpo Mouse, Rat)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Rat CENP-O with 11 mouse-specific point mutations…

    Promoter

    T7

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MAC-N SARS-CoV-2 Omicron spike protein

    Plasmid

    #180779

    Purpose

    Sars-CoV-2 variant protein

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    MAC-N SARS-CoV-2 Omicron spike protein
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    S371L, H69-V70-, S373P, S375F, G339D, N211-, N440…

    Promoter

    Available since

    Sept. 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. ERAP1-rs27895(T)

    Plasmid

    #206143

    Purpose

    Expresses the rs27895-T allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mutation

    G346 changed to D346

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid

    #197267

    Purpose

    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Insert

    NEAT1-total homology arms
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pINSPECT_SigP:NLuc

    Plasmid

    #197263

    Purpose

    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-noSV40-humanEC1.45_p300peak1-2_del11

    Plasmid

    #173983

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #11
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Tiled deletion #11

    Promoter

    SV40 promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge