We narrowed to 50,243 results for: ANG

1. MO91-dCas9-AID(P182X)

   Plasmid

   #83260

   Purpose

   Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

   Depositor

   Xing Chang

   Article

   Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

   Insert

   dCas9-AID P182X
   

   Use

   Retroviral

   Tags

   3xFLAG-NLS and FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9; P182X in AID

   Promoter

   MSCV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pW5Y_Apr

   Plasmid

   #158211

   Purpose

   BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

   Plasmid

   #192068

   Purpose

   Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Ctip2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-Dronpa

   Plasmid

   #87709

   Purpose

   FKBP-tagged Dronpa.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FKBP-Dronpa
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-Ubi-miniSdd7-nCas9-PBE

   Plasmid

   #204856

   Purpose

   For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1, OsU3, 35S

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLNCX2-NGN2-IRES-GFP

   Plasmid

   #90212

   Purpose

   To convert human fetal lung fibroblasts into induced cholinergic neurons (hiCN) in combination with two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP (NEUROG2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dronpa-DEVD-TagRFPT

   Plasmid

   #87704

   Purpose

   Fluorescent biosensor for super-resolution imaging of caspase activity.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   Dronpa-DEVD-TagRFPT
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

   Plasmid

   #159756

   Purpose

   PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

   Plasmid

   #192067

   Purpose

   Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for human FXOP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CMV-miniSdd7-BE4max-BlastR

    Plasmid

    #204851

    Purpose

    For cytosine base editing using miniSdd7 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNSSCP

    Plasmid

    #183188

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgNCKAP1-2

    Plasmid

    #210134

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-sgNCKAP1-1

    Plasmid

    #210133

    Purpose

    knock out NCKAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Nck-associated protein 1 (NCKAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 myc card9 mutant

    Plasmid

    #16254

    Depositor

    William Kaelin

    Article

    Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

    Insert

    card9 mutant (CARD9 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S528A, T531A, T533A, S536A

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1+-PAFAH1B1_R22-

    Plasmid

    #92431

    Purpose

    mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R22 (stop codon in position 22)

    Promoter

    CMV

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-U6-pegRNA_KCNA1-EGFP

    Plasmid

    #159787

    Purpose

    S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge