We narrowed to 3,528 results for: MAK;

1. SiRES COR1C ACT--GFP

   Plasmid

   #195151

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SiRES COR1C ACT--Halo

   Plasmid

   #195152

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-ATG101

   Plasmid

   #189590

   Purpose

   To make ATG101/ATG13 HORMA complex

   Depositor

   James Hurley

   Article

   Shi et al J Cell Biol. 2020 Jul 6;219(7):e20191104

   Insert

   ATG101 (ATG101 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NeuA.pJL1

   Plasmid

   #199115

   Purpose

   In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

   Depositor

   Michael Jewett

   Article

   Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

   Insert

   N-acylneuraminate cytodiylyltransfease (NeuA)
   

   Use

   Tags

   Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYL237

   Plasmid

   #173194

   Purpose

   CRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 RNA-binding mutant
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSARN-vasa-eSpCas9sv40

   Plasmid

   #173670

   Purpose

   eSpCas9 expression vector for Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Insert

   eSpCas9 with Anopheles vasa promoter and SV40 terminator
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   mutations in Cas9 reducing off-target activity (S…

   Promoter

   vasa

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-myc-Sema4A RNAiR

   Plasmid

   #115172

   Purpose

   expresses RNAi-resistant Sema4A with N-terminal myc tag

   Depositor

   Suzanne Paradis

   Article

   McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

   Insert

   Sema4A (Sema4a Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Silent mutation between amino acids in ORF (401-4…

   Promoter

   CMV

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-6NBC-GFP-PGK-Puro-Barcode81

   Plasmid

   #83758

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode81
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   deletion of T in starting ATG of GFP (please see …

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-6NBC-GFP-PGK-Puro-Barcode71

   Plasmid

   #83748

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode71
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   deletion of AA5-233 in GFP (please see depositor …

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 and H1

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-V5-KDM3A

    Plasmid

    #82331

    Purpose

    lentiviral expression of KDM3A

    Depositor

    Luke Gaughan

    Article

    Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

    Insert

    KDM3A (KDM3A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    two silent mutations to make it siRNA resistant

    Promoter

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN-mCherry-Parkin

    Plasmid

    #59419

    Purpose

    to make mCherry-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    mCherry-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJM1-FLAG-metap2

    Plasmid

    #100510

    Purpose

    Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    METAP2 (METAP2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH4

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. modRNAc0-ABE8e-P2A-GFP

    Plasmid

    #178177

    Purpose

    IVT template plasmid for making ABE8e-P2A-GFP modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    ABE8e
    

    Use

    Ivt template plasmid

    Tags

    P2A-GFP

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. neurod1:cre; cmlc2:EGFP

    Plasmid

    #173480

    Purpose

    cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Cre
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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