We narrowed to 21,779 results for: his

1. CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Plasmid

   #200798

   Purpose

   Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   411-680

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

   Plasmid

   #227611

   Purpose

   Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   MBP, 10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-88 only

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

   Plasmid

   #227089

   Purpose

   Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)

   Depositor

   Chris Hill

   Insert

   tom1p-HECT (TOM1 Budding Yeast)
   

   Use

   Tags

   HIS-MBP-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EV-D68 2A protease; 6xHis-SUMO, low expression levels

   Plasmid

   #204802

   Purpose

   Bacterial expresison of Enterovirus D68 2A protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   D68 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

   Plasmid

   #217329

   Purpose

   Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S729A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

   Plasmid

   #217325

   Purpose

   Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   N61K

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

   Plasmid

   #217324

   Purpose

   Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid

    #217320

    Purpose

    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

    Plasmid

    #221891

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

    Plasmid

    #221890

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    HNRNPA3 (HNRNPA3 Human)
    

    Use

    Tags

    6xHis-MBP-PreScission-mGFP-TEV

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

    Plasmid

    #211890

    Purpose

    Bacterial expression of CENP-T[1-242/3D]-I3-01

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T11D, T27D, T85D

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

    Plasmid

    #211891

    Purpose

    Bacterial expression of CENP-T[1-242/3D]

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242/3D] (CENPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T11D, T27D, T85D

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

    Plasmid

    #212795

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo(K48R/K63R)-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    CUb(K48R/K63R)

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmGIGYF_1-640-del331-370-V5His6_AH

    Plasmid

    #148906

    Purpose

    Insect Expression of DmGIGYF_1-640-del331-370

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_1-640-del331-370 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

    Plasmid

    #148623

    Purpose

    Bacterial Expression of DmRoquin_702-819-del790-812

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_702-819-del790-812 (roq Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE

    Plasmid

    #148622

    Purpose

    Bacterial Expression of DmRoquin_702-819-del725-755

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_702-819-del725-755 (roq Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z

    Plasmid

    #148157

    Purpose

    Insect Expression of Dm4E-T_1-511-del28-43

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-del28-43 (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two non silent mutations L127S, annotated as EST,…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

    Plasmid

    #148158

    Purpose

    Insect Expression of Dm4E-T_1-511-FWKtoAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-FWKtoAAA (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two non silent mutations L127S, annotated as EST,…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z

    Plasmid

    #148161

    Purpose

    Insect Expression of Dm4E-T_1-511-Y10AL15A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-Y10AL15A (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two non silent mutations L127S, annotated as EST,…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z

    Plasmid

    #148162

    Purpose

    Insect Expression of Dm4E-T_1-511-YLLLtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-511-YLLLtoAAAA (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two non silent mutations L127S, annotated as EST,…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T

    Plasmid

    #147600

    Purpose

    Insect Expression of Dm4E-T_1-510-Y10AL15A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-510-Y10AL15A (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pnEA-NpM-HsNot4_275-575-del377-402-GB1His6_AG

    Plasmid

    #148785

    Purpose

    E. coli of HsNot4_275-575-del307-402

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_275-575-del307-402 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pnEA-NpM-HsNot4_275-575-del377-424-GB1His6_AG

    Plasmid

    #148786

    Purpose

    Bacterial Expression of HsNot4_275-575-del377-424

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_275-575-del377-424 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pnEA-NpM-HsNot4_275-575-del400-428-GB1His6_AG

    Plasmid

    #148787

    Purpose

    E. coli of HsNot4_275-575-del400-428

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_275-575-del400-428 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_AC

    Plasmid

    #148467

    Purpose

    Insect Expression of DmNanos_116-163-F152E

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_116-163-F152E (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_AC

    Plasmid

    #148468

    Purpose

    Insect Expression of DmNanos_116-163-L127DF130D

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_116-163-L127DF130D (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-lambdaN-HA-DmCup_1-417-4Ala-mut-V5His6_M

    Plasmid

    #146897

    Purpose

    Insect Expression of DmCup_1-417-4Ala-mut

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_1-417-4Ala-mut (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L

    Plasmid

    #146862

    Purpose

    Insect Expression of DmPABPC1_del183-258-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    DmPABPC1_del183-258-siRNAres (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation G26S, and seven silent mu…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge