We narrowed to 29,109 results for: PLE;

1. pAW211

   Plasmid

   #113233

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GGTAG->AACGA

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW210

   Plasmid

   #113189

   Purpose

   E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H208A

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAW216

   Plasmid

   #113238

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GGTAG->ggtTg

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW203

   Plasmid

   #113243

   Purpose

   E. coli expression vector for IHFa E10A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa E10A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E10A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRec-GalS

   Plasmid

   #104573

   Purpose

   Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively.

   Depositor

   Harris Wang

   Article

   Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

   Insert

   PTet-cas12, tetR, lacI-galS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRec-TreR

   Plasmid

   #104574

   Purpose

   Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.

   Depositor

   Harris Wang

   Article

   Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

   Insert

   PTet-cas12, tetR, lacI-treR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWp9-2

   Plasmid

   #73853

   Purpose

   pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   UBCp-RFP-CMVp-GFP-U6p-RFP-sg1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pJexpress414_OmpA171_R4cons_Y129H

   Plasmid

   #64302

   Purpose

   Conservative version of OmpA Redesign 4 with Y129H mutation

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_R4cons_Y129H
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   25 mutations to lipid-facing surface

   Promoter

   T7

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUGW-H1-Scrambled control shRNA

    Plasmid

    #40625

    Depositor

    Sally Temple

    Article

    Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

    Insert

    Scrambled control shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP1183

    Plasmid

    #104163

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cyto-V5-APEX2

    Plasmid

    #170572

    Purpose

    Expresses APEX2 in cytosol

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. scAAV-hIBA1a-GFP

    Plasmid

    #214146

    Purpose

    Self-complementary AAV vector packaging GFP under the human Iba1 promoter

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK232 (CMV-OsTIR1-PURO)

    Plasmid

    #72834

    Purpose

    Expresses OsTIR1 under the control of the CMV promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-CMV-OsTIR1-PURO-AAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330S-2

    Plasmid

    #58778

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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