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pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180590

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Tags

Expression

Mutation

PHR(E281A)

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183519

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Has Service

AAV8

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pXD043 KIKO CD22 CAR AAV vector

Plasmid

#192158

Purpose

CD22 CAR AAV vector

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

anti-CD22 CAR (CD22 Human)

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CAG-hM4Di-P2A-dTomato

Plasmid

#202037

Purpose

AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

Depositor

Peter Jonas, Yoav Ben-Simon

Insert

hM4Di

Use

AAV

Tags

dTomato

Expression

Mutation

Promoter

CAG

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mPGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS654 All-in-One AAV-sgRNA-hNmeCas9

Plasmid

#112139

Purpose

Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

Depositor

Erik Sontheimer

Article

Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

Inserts

sgRNA scaffold
Human-codon-optimized NmeCas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U1a and U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Expression

Mutation

Promoter

CamK II

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RHO-IntC-dCas9C-VPR-polyA

Plasmid

#166692

Purpose

Express C-terminal part of split dCas9-VPR

Depositor

Stylianos Michalakis

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Insert

IntC-dCas9C-VPR

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Tags

Expression

Mutation

Promoter

hGFAP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Expression

Mutation

H134R

Promoter

TRE

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

Plasmid

#211818

Purpose

AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

C-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter and U6 promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Robert Malenka, Kevin Beier

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Tags

Expression

Mutation

NA

Promoter

hSyn

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff DREADD Gq-mCherry

Plasmid

#183533

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Tags

Expression

Mutation

None

Promoter

nEF

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

Plasmid

#104491

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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