We narrowed to 5,111 results for: codon optimized

1. pDF0317 HvsCas7-11 mammalian expression

   Plasmid

   #172511

   Purpose

   Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   HvsCas7-11 mammalian expression
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. P25-pZE-SUMO-PbTTA

   Plasmid

   #204634

   Purpose

   ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

   Depositor

   Aditya Kunjapur

   Article

   Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

   Insert

   His-SUMO-TEV-PbTTA
   

   Use

   Synthetic Biology

   Tags

   His Tag; SUMO Tag; TEV Protease Site

   Expression

   Bacterial

   Promoter

   PLtetO-1 promoter

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3cH_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104912

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 HA-FAM208A

   Plasmid

   #115845

   Purpose

   Encodes codon optimized HA tagged FAM208A

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HA tagged FAM208A (C3orf63 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV306DIG13+FFlucRT

   Plasmid

   #49998

   Purpose

   Integrase removed to increase stability of the plasmid in mycobacteria

   Depositor

   Brian Robertson

   Article

   Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

   Insert

   G13 promoter + Firefly luciferase Red Thermostable
   

   Use

   Mycobacteria integrating vector

   Expression

   Bacterial

   Mutation

   Codon optimized for Mycobacterium tuberculosis wi…

   Promoter

   G13

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP469

   Plasmid

   #65771

   Purpose

   Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEND-227_Cacnes_mCerulean

   Plasmid

   #225614

   Purpose

   Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCerulean
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cK_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104908

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGB 35s:dCas:BRD:tNos (GB1172)

    Plasmid

    #75401

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas9:BRD
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

    Plasmid

    #201551

    Purpose

    Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Kinesin-1 (partial)
    

    Tags

    HaloTag

    Expression

    Insect

    Mutation

    Codon-optimized for Drosophila expression

    Promoter

    Ac5

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Expression

    Mammalian

    Mutation

    T205I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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