We narrowed to 12,328 results for: pcdna

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pcDNA4/TO-ORF24-3xFLAG

Plasmid

#138423

Purpose

Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-Zmat3R99Q

Plasmid

#167344

Purpose

Expresses HA-tagged R99Q mutant Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

Zinc finger matrin-type 3 (Zmat3 Mouse)

Tags

3HA

Expression

Mammalian

Mutation

changed arginine 99 to glutamine

Promoter

CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-D176H

Plasmid

#69919

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

D176H

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L198A

Plasmid

#8975

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Trib1

Plasmid

#131156

Purpose

Mammalian expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ORF24-2xStrep pCDNA4

Plasmid

#129742

Purpose

Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF24 (ORF24 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF66-2xStrep

Plasmid

#130953

Purpose

Expresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 1-429 (full-length)

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL50

Plasmid

#135101

Purpose

Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gα13(EE)-YFP

Plasmid

#171374

Purpose

Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Ga13(EE)-YFP

Tags

Glu-Glu

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-GATA2-DC+Nzf

Plasmid

#1293

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA6-chCAR-MycHis

Plasmid

#87232

Purpose

Mammalian expression of chicken CAR

Depositor

Fritz Rathjen

Insert

CXADR (CXADR Chicken)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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GET1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176985

Purpose

Mammalian expression vector encoding GET1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GET1 (GET1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mEGFP

Plasmid

#168490

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP

Expression

Mammalian

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB2b

Plasmid

#19470

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2b (DNAJB2 Human)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp7

Plasmid

#157689

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp7 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Myc-gamma3 (in pCDNA3.1)

Plasmid

#118041

Purpose

GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG3 (Gabrg3 Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-gamma1 (in pCDNA3.1)

Plasmid

#118038

Purpose

GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG1 (Gabrg1 Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nAChr-beta3

Plasmid

#48811

Purpose

This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

Depositor

Henry Lester, Jerry Stitzel

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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