We narrowed to 5,408 results for: U6

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pCRISPR/eCas9-GPIDAF

Plasmid

#213710

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211688

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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HypaR-SpCas9

Plasmid

#126757

Purpose

Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v2 (opti)

Plasmid

#177250

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_2nd

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNT3 (opti)

Plasmid

#177239

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT3

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo1 (opti)

Plasmid

#177240

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo2 (opti)

Plasmid

#177241

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgNeo3 (opti)

Plasmid

#177242

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNeo3

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgLkb1_IW (opti)

Plasmid

#177243

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Lkb1 gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgLkb1_v1

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-sgLkb1_v2 (opti)

Plasmid

#177244

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Lkb1 gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgLkb1_v2

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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