Skip to main content
Addgene
Showing: 6681 - 6720 of 8371 results

  1. AAV-sCAG-eGFP-RAB11

    Plasmid
    #203799
    Purpose
    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203799-plasmid-map-sequence-id-413679

    Enlarge

  3. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

    Enlarge

  4. FLT3-ITD-MSCV-IRES-GFP

    Plasmid
    #184779
    Purpose
    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating internal tandem duplication (ITD) adde…
    Promoter
    MSCV
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184779-plasmid-map-sequence-id-364900

    Enlarge

  5. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC

    Plasmid
    #221355
    Purpose
    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221355-plasmid-map-sequence-id-436811

    Enlarge

  6. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid
    #221357
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221357-plasmid-map-sequence-id-436816

    Enlarge

  7. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

    Enlarge

  8. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114404-plasmid-map-sequence-id-220815

    Enlarge

  9. pAAV/hSyn-SCLM

    Plasmid
    #216760
    Purpose
    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    hSyn (human synapsin I)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216760-plasmid-map-sequence-id-429492

    Enlarge

  10. 7MEQ

    Plasmid
    #176412
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    8 x His Tag and Honeybee melittin signal
    Expression
    Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176412-plasmid-map-sequence-id-345854

    Enlarge

  11. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

    Enlarge

  12. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid
    #199781
    Purpose
    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
    Depositor
    Sascha Martens
    Insert
    NEMO (IKBKG Human)
    Use
    Tags
    GST-Thrombin-TEV-EGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199781-plasmid-map-sequence-id-418258

    Enlarge

  14. pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

    Plasmid
    #221365
    Purpose
    Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Rho IPI-[1D4] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221365-plasmid-map-sequence-id-436834

    Enlarge

  17. AICSDP-166:CDH1-mEGFP

    Plasmid
    #193921
    Purpose
    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1
    Depositor
    Allen Institute for Cell Science
    Insert
    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    Use
    AAV and CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193921-plasmid-map-sequence-id-380537

    Enlarge

  21. Galt1-Grx1-roGFP1-iE

    Plasmid
    #230029
    Purpose
    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230029-plasmid-map-sequence-id-458346

    Enlarge

  22. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid
    #205151
    Purpose
    Express UbC-driven LARGE1 and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205151-plasmid-map-sequence-id-405996

    Enlarge

  23. Mouse 3' Hp1a AID GFP PuroR

    Plasmid
    #127897
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene
    Depositor
    Mark Groudine
    Insert
    Hp1a (Cbx5 Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127897-plasmid-map-sequence-id-250990

    Enlarge

  24. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107580-plasmid-map-sequence-id-208159

    Enlarge

  25. pET28a-UBC9-K14R-A129K

    Plasmid
    #228948
    Purpose
    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Mutation
    changed Lysine 14 to Arginine, changed Alanine 12…
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228948-plasmid-map-sequence-id-472389

    Enlarge

  26. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

    Enlarge

  27. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

    Enlarge

  33. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid
    #50477
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50477-plasmid-map-sequence-id-370683

    Enlarge

  34. Membrane mNeonGreen

    Plasmid
    #198057
    Purpose
    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane mNeonGreen (LCK Human, A.victoria)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mNeonGreen
    Expression
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198057-plasmid-map-sequence-id-389150

    Enlarge

  35. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid
    #184780
    Purpose
    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating tyrosine kinase domain mutation (D835Y)
    Promoter
    MSCV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184780-plasmid-map-sequence-id-367916

    Enlarge

Showing: 6681 - 6720 of 8371 results