We narrowed to 10,333 results for: SEC

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TPST1-EGFP

Plasmid

#66617

Purpose

Encodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Article

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST1 (TPST1 Human)

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-Munc18-1wt

Plasmid

#135550

Purpose

Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18-1wt

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hGFAP-Pink Flamindo-WPRE-SV40

Plasmid

#178790

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

Pink Flamindo

Use

AAV

Promoter

hGFAP

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mH2A1.2-CT-GFP

Plasmid

#45169

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTU2S-a (p15A origin)

Plasmid

#74088

Purpose

Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Expression

Mammalian

Mutation

Q71L

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

Plasmid

#231204

Purpose

ER-retained mouse renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

Plasmid

#231203

Purpose

ER-retained H2M renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hHus1

Plasmid

#222470

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Tags

Flag epitope

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-IL3-218-PDGFR

Plasmid

#139055

Purpose

A lentivirus vector to integrate a surface membrane anchored human IL3 gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

interleukin 3(IL3) (IL3 Human)

Use

Lentiviral

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKC52

Plasmid

#226780

Purpose

Expresses Drosophila UNC-104(R348G) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R348G) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 348 to Glycine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC50

Plasmid

#226778

Purpose

Expresses Drosophila UNC-104(V6M) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (V6M) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Valine 6 to Methionine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC28

Plasmid

#226776

Purpose

Expresses Drosophila UNC-104(G618R) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (G618R) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

G618R

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mucin MUC1-5TR-pET15b

Plasmid

#174694

Purpose

Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

Depositor

Jean Gariepy

Article

Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

Insert

MUC1-TR

Tags

hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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