We narrowed to 2,365 results for: DAP

1. pAAV-MCHpr-DREADD (Gq)-mCherry

   Plasmid

   #221036

   Purpose

   Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

   Depositor

   Yanhua Huang

   Article

   Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV; Rat targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rat MCH

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Blast-V5-LMNB1

   Plasmid

   #207779

   Purpose

   Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. qTAG-C-Solo-mScarlet-H3C2

   Plasmid

   #207781

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. qTAG-C-mNeon-Blast-H3C2

   Plasmid

   #207782

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Blast-moxGFP-TUBA1B

   Plasmid

   #207766

   Purpose

   Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGADT7-δ

   Plasmid

   #197259

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-3 δ (AP3D1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Val 549 Ile substitution; silent substitutions in…

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBridge-tyrosinase.σ3A

   Plasmid

   #197513

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-3 σ3A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   silent substitution in codon 2 of tyrosinase tail…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas_4(I-U)/1_2_VB

   Plasmid

   #186445

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain swapped with Cas4 domain from G. sulf…

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGADT7-ε

   Plasmid

   #197261

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-4 ε (AP4E1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitutions in codons 905, 1129 and 1137

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. S+Q111R

    Plasmid

    #167173

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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