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pGEX4T1_GST-EGFP-ATG13_IDR_delta191-230 (231-517aa)

Plasmid

#223785

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 231-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa)

Plasmid

#223786

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 205-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

Plasmid

#223787

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with 206-230 deleted

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223789

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Mutation

E72A/L75A/D77A/E81A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI4

Plasmid

#223769

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI4 (WDR45 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

Plasmid

#223738

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-SAM-Puro

Plasmid

#198713

Purpose

For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Inserts

U6-MS2-gRNAscaffold
EF1a-MPH-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-SAM-Cre

Plasmid

#198714

Purpose

For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Inserts

U6-MS2-gRNAscaffold
Pgk-MPH-Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

Pgk and U6

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/26R]

Plasmid

#225386

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 [L51/73R]

Plasmid

#225373

Purpose

Mammalian Expression Plasmid of anti-MMP9 (Rat) IgG2a R-mAb. Derived from hybridoma L51/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-MMP9 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Mmp9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-NALCN [N185/7R-2b]

Plasmid

#225412

Purpose

Mammalian Expression Plasmid of anti-NALCN (Rat) IgG2b R-mAb. Derived from hybridoma N185/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-NALCN (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Nalcn Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/44R]

Plasmid

#225387

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/44.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Synaptotagmin-6 [N270/28R]

Plasmid

#225385

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse) IgG2a R-mAb. Derived from hybridoma N270/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-6 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Synaptotagmin-3 [N278/16R]

Plasmid

#225388

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-3 (Mouse) IgG2a R-mAb. Derived from hybridoma N278/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-3 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/15R]

Plasmid

#225379

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat) IgG2a R-mAb. Derived from hybridoma N121A/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Pannexin-2 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Panx2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Neuroligin-1 [N495/90R]

Plasmid

#225400

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat) IgG2a R-mAb. Derived from hybridoma N495/90.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-1 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Nlgn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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