We narrowed to 7,345 results for: Lif;

1. pCMV superYFP-AURKA K162M-mTurq2

   Plasmid

   #157773

   Purpose

   Expression of AuroraA kinase-dead biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2 kif26b sgRNA1

   Plasmid

   #102857

   Purpose

   Expresses sgRNA targeting mouse Kif26b

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Kif26b (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2 kif26b sgRNA2

   Plasmid

   #102858

   Purpose

   Expresses sgRNA targeting mouse Kif26b

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Kif26b (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-PDGFRA-P577S-V5/HIS

   Plasmid

   #234760

   Purpose

   Expression of the P577S mutant variant of human PDGFRA, which might be associated with Melanoma

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   P577S substitution

   Promoter

   CMV

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-PDGFRA-R487L-V5/HIS

   Plasmid

   #234761

   Purpose

   Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   R487L substitution

   Promoter

   CMV

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-PDGFRA-G853D-V5/HIS

   Plasmid

   #234757

   Purpose

   Expression of the G853D mutant variant of human PDGFRA, which might be associated with Melanoma

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-G853D receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   G853D substitution

   Promoter

   CMV

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-PDGFRA-T674I-V5/HIS

   Plasmid

   #234756

   Purpose

   Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   T674I substitution

   Promoter

   CMV

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-PDGFRA-K627M-V5/HIS

   Plasmid

   #234755

   Purpose

   Expression of the K627M kinase defective mutant variant of human PDGFRA

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   K627M substitution

   Promoter

   CMV

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEW108

   Plasmid

   #232823

   Purpose

   Expresses yeast compass complex in insect cells

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

   Insert

   COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
   

   Use

   Tags

   His6-3xFLAG, Twinstrep

   Expression

   Insect

   Mutation

   Set1(762-1080)

   Promoter

   polyhedrin promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG CAPN7 V18D,F98D-OSF

    Plasmid

    #193045

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D, F98D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA528 IST1 L328D (316-366)(C-Cys)

    Plasmid

    #193035

    Purpose

    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, Residues 316-366, Non-native C-terminal Cy…

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA528 IST1 L355A (316-366)(C-Cys)

    Plasmid

    #193036

    Purpose

    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L355A, Residues 316-366, Non-native C-terminal Cy…

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CHMP1B 143-199

    Plasmid

    #108255

    Purpose

    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1B residues 143-199 (CHMP1B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 CHMP4B 156-224

    Plasmid

    #104596

    Purpose

    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B residues 156-224 (CHMP4B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRXIP-GFP-0K9Q

    Plasmid

    #221743

    Purpose

    Stable expression of GFP-0K9Q in mammalian cells to monitor membrane charge

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    C -terminal region of KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-170, mutated K to Q

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP-GFP-1K8Q

    Plasmid

    #221742

    Purpose

    Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    C -terminal region of KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-170, mutated K to Q

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRXIP-GFP-3K6Q

    Plasmid

    #221741

    Purpose

    Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    C -terminal region of KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-170, mutated K to Q

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRXIP-GFP-5K4Q

    Plasmid

    #221740

    Purpose

    Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    C -terminal region of KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-170, mutated K to Q

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid

    #180641

    Purpose

    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B (CHMP4B Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 156-224

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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