We narrowed to 3,806 results for: golden gate

1. pMK003

   Plasmid

   #236570

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK025

   Plasmid

   #236572

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFlp-Gm

   Plasmid

   #222335

   Purpose

   Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Promoter

   cl, flp, and sacB

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM-mTagBFP2

   Plasmid

   #228615

   Purpose

   Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB90 - pAGM4723_VirGN54D

   Plasmid

   #123187

   Purpose

   Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Type

   Empty backbone

   Use

   Level 2 acceptor vector for expression in plants

   Expression

   Plant

   Mutation

   VirGN54D for increase virulence

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR_attB(bxb)_ccdB_lox

   Plasmid

   #183763

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ccdB
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS1871

    Plasmid

    #205976

    Purpose

    U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH DR-BpiI-DR
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGT6471

    Plasmid

    #219428

    Purpose

    Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
    

    Use

    Moclo compatible level 0 module

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN-iRS3GG

    Plasmid

    #98858

    Purpose

    Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

    Depositor

    Lydia Contreras

    Article

    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

    Insert

    PheS cassette to be selectively replaced by unique asRNA probes
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK024

    Plasmid

    #236571

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISAam1 guides into ISAam1 plant expression vector

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNP2987 [pNP2922 - ISCro4 TBL3 (WT) + ISCro4 Recombinase]

    Plasmid

    #247256

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA35-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL3)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. L4278 hIgKLSS_hTGFb1 in pcDNA backbone

    Plasmid

    #244184

    Purpose

    Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    TGF-beta1 with IgK light chain secretion sequence (TGFB1 Synthetic, Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. L4275 hIgKLSS_hTNF in pcDNA backbone

    Plasmid

    #244182

    Purpose

    Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    TNF with IgK light chain secretion sequence (TNF Synthetic, Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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