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Showing: 801 - 850 of 1383 results

  2. P-d22bp

    Plasmid
    #235657
    Purpose
    B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site
    Depositor
    Guy Townsend
    Article
    Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.
    Insert
    Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site
    Use
    Tags
    Expression
    Mutation
    The B. thetaiotaomicron fusA2 promoter lacking &q…
    Promoter
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235657-plasmid-map-sequence-id-474231

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  3. pNDGG059

    Plasmid
    #231325
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Tc-par-ELT4; GG assembly from pOGG004, pOGG042, pNDGG005, pOGG012, and pOGG014, TcR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231325-plasmid-map-sequence-id-471990

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  5. pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB

    Plasmid
    #225488
    Purpose
    Tet-on lentiviral vector for controlled expression of GDNF
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse phosphoglycerate kinase 1
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225488-plasmid-map-sequence-id-444525

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  11. pSW002-Pc-mRuby2

    Plasmid
    #111250
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mRuby2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mRuby2 is codon optimized for expression in P. fl…
    Promoter
    Pc
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111250-plasmid-map-sequence-id-215255

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  13. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

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  21. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid
    #164255
    Purpose
    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid
    Depositor
    John Maciejowski
    Article
    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2
    Insert
    TRF2
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    deletion of amino acids-86 and 496-542
    Promoter
    Tetracycline inducible promotor
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    164255-plasmid-map-sequence-id-329101

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  22. pWJB9

    Plasmid
    #126650
    Purpose
    Expresses AdrA from a modified pLAFR3
    Depositor
    Ute Romling
    Article
    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d
    Insert
    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126650-plasmid-map-sequence-id-252591

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  23. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

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  24. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  28. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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  29. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  33. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  35. pYPQ132-tRNA2.0

    Plasmid
    #158394
    Purpose
    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158394-plasmid-map-sequence-id-330366

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  40. pYPQ133-tRNA2.0

    Plasmid
    #158395
    Purpose
    Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158395-plasmid-map-sequence-id-330367

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  41. pYPQ134B2.0

    Plasmid
    #167158
    Purpose
    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167158-plasmid-map-sequence-id-331276

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  42. pPRIME-TET-GFP-FF3

    Plasmid
    #11662
    Purpose
    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
    Depositor
    Stephen Elledge
    Article
    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1
    Insert
    FF3
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    11662-plasmid-map-sequence-id-7008

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  43. pYPQ134-tRNA2.0

    Plasmid
    #158396
    Purpose
    Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158396-plasmid-map-sequence-id-330816

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  44. pYPQ135-tRNA2.0

    Plasmid
    #158397
    Purpose
    Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158397-plasmid-map-sequence-id-330905

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  45. pYPQ136-tRNA2.0

    Plasmid
    #158398
    Purpose
    Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158398-plasmid-map-sequence-id-330906

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  47. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-tubulin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

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  48. pYPQ135B2.0

    Plasmid
    #167159
    Purpose
    Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167159-plasmid-map-sequence-id-331281

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  50. pAOJ15

    Plasmid
    #91567
    Purpose
    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline promoter
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    91567-plasmid-map-sequence-id-181626

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Showing: 801 - 850 of 1383 results