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pXSE901BG

Plasmid

#91714

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHEE901

Plasmid

#91707

Purpose

CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

Plasmid

#228727

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

Plasmid

#228728

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D56R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

Plasmid

#228733

Purpose

Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid eNme-C nCas9 (D16A)-i1-L10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHSE901

Plasmid

#91708

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-eA3Amax-BlastR

Plasmid

#152997

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABE8eHD-BlastR

Plasmid

#176478

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABE8eHD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHMattR3-attR4-GFP

Plasmid

#196247

Purpose

pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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CP1028-CBEmax

Plasmid

#119802

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1028) -UGI-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pnxABE-flgn

Plasmid

#187932

Purpose

A-to-G base editor

Depositor

Yi Xiao

Article

et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

Insert

ecTadA(8e)-nxCas9 (D10A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CP1041-ABEmax

Plasmid

#119808

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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VRER-ABEmax

Plasmid

#119812

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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CP1041-CBEmax

Plasmid

#119803

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1041) -UGI-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1249-ABEmax

Plasmid

#119809

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBSE901

Plasmid

#91709

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1012-ABEmax

Plasmid

#119806

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge