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pBiT3.1-N_NLE1:1-485

Plasmid

#211141

Purpose

Mammalian expression of full-length human NLE1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NLE1:M1-R485 (NLE1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NLE1:1-485

Plasmid

#211182

Purpose

Mammalian expression of full-length human NLE1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NLE1:M1-R485 (NLE1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural v…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-actin

Plasmid

#118378

Purpose

Gateway compatible donor vector with WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
MAC-CTSL

Plasmid

#172414

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Procathepsin L (Cathepsin L1) (CTSL Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3XFlag pCMV5-TOPO TAZ (S89A)

Plasmid

#24815

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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3XFlag pCMV5-TOPO TAZ (∂WW)

Plasmid

#24811

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 111-158.

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ (∂304)

Plasmid

#24810

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-304.

Available Since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ (106∂)

Plasmid

#24814

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 1-106

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCW57-CMV-MAAP-SL08

Plasmid

#218247

Purpose

1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Available Since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
MAC-TMPRSS4

Plasmid

#172422

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 4 (TMPRSS4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTE3987

Plasmid

#137716

Purpose

Expresses dLbCas12a-RVRR-BE in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dLbCas12a-RVRR-BE

Tags

6xHis, APOBEC-1, SV40 NLS, and UGI

Expression

Mammalian

Mutation

G532A, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP-SL08V9

Plasmid

#218251

Purpose

1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP-SL01

Plasmid

#218248

Purpose

1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDON223 Spike D138Y

Plasmid

#180056

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D138Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D138Y

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A570D

Plasmid

#180064

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A570D (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A570D

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike H655Y

Plasmid

#180059

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike H655Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike H655Y

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike S982A

Plasmid

#180067

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike S982A (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike S982A

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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