We narrowed to 4,139 results for: chm

1. pTNFR1-tdEOS

   Plasmid

   #98273

   Purpose

   mammalian expression of TNFR1-tdEOS

   Depositor

   Mike Heilemann

   Article

   Heidbreder et al Biochim Biophys Acta. 2012 Oct;1823(10):

   Insert

   TNFR1 (TNFRSF1A Human)
   

   Use

   Tags

   Flag and tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-U6-sgRNA-BFP-Puro

   Plasmid

   #229014

   Purpose

   Perturb Seq lentiviral sgRNA vector

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACT1:Cas9-GFP, U6:sgTK

   Plasmid

   #122852

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgTK
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. UPRT-mCh-Nluc-P2A-neo-UPRT

   Plasmid

   #135015

   Purpose

   Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   mCh-Nluc-P2A-neo
   UPRT 5'UTR
   UPRT 3'UTR
   

   Use

   Cryptosporidium expression

   Tags

   mCherry and Nluc-neo

   Expression

   Mutation

   Promoter

   Cryptosporidium actin and enolase

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Donor-attp-u6-blank-sgrna-backbone

   Plasmid

   #234076

   Purpose

   Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   sgRNA-Blank
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-DIO-PinkyCaMP

   Plasmid

   #232857

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-SFFV-GLuc-CreERT2-mCherry

   Plasmid

   #178185

   Purpose

   the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

   Depositor

   Irmela Jeremias

   Article

   Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

   Inserts

   GLuc
   CreERT2
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-SA-CAG-Pa01-BFP

   Plasmid

   #234142

   Purpose

   Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   Pa01
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA52_Lenti.puro.U6_modified DR

    Plasmid

    #196723

    Purpose

    For cloning pairs of AsCas12a guides with two DRs that are as different as possible.

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BFP-U6-sgRNA

    Plasmid

    #196725

    Purpose

    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPSeq-BFP

    Plasmid

    #196718

    Purpose

    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-TRAF1

    Plasmid

    #111199

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    TRAF1 (TRAF1 Human)
    

    Use

    Tags

    EGFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EYFP-TRAF1

    Plasmid

    #111200

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    TRAF1 (TRAF1 Human)
    

    Use

    Tags

    EYFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ECFP-TRAF1

    Plasmid

    #111198

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    TRAF1 (TRAF1 Human)
    

    Use

    Tags

    ECFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid

    #196724

    Purpose

    Sortable and selectable EnAsCas12a.

    Depositor

    Bernhard Schmierer

    Insert

    enASCas12a-HA-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS - HA and SV40 NLS

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-AR

    Plasmid

    #111215

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    AR (Ar Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge