We narrowed to 29,740 results for: REP

1. pRetroX-TRE3G_NT-GSDMD_C39A

   Plasmid

   #201171

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa1-276 only, C39A

   Promoter

   TRE3G

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcmv3-IRG1-H277Y-HA

   Plasmid

   #198185

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The histidine in IRG1 at position 277 mutates to …

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-MYO10-FERM-ITGBD

   Plasmid

   #194855

   Purpose

   Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

   Insert

   MYO10 S2001_F2002insA/T2009D (MYO10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   MYO10 has the following mutations S2001_F2002insA…

   Promoter

   CMV

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

   Plasmid

   #197891

   Purpose

   Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   ChRWR/Venus
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNHT7::UMSBP::sNluc2_ORF1

   Plasmid

   #186765

   Purpose

   An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNHT7::ENO::sNluc2

   Plasmid

   #186770

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNHT7::RPL15::sNluc0

   Plasmid

   #186771

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNHT7::RPL15::hNluc

   Plasmid

   #186772

   Purpose

   An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNHT7::YB1::hNluc

   Plasmid

   #186774

   Purpose

   An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAPDH::sNluc1

    Plasmid

    #187221

    Purpose

    A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Overexpression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNHT7::YB1::sNluc1

    Plasmid

    #186754

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNHT7::ENO::sNluc1

    Plasmid

    #186755

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNHT7::RPL10::sNluc1

    Plasmid

    #186756

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNHT7::RPL15::sNluc1

    Plasmid

    #186757

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNHT7::YB1::sNluc0

    Plasmid

    #186758

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNHT7::YB1::sNluc2

    Plasmid

    #186759

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNHT7::YB1::sNluc3

    Plasmid

    #186760

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNHT7::YB1::sNluc4

    Plasmid

    #186761

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNHT7::YB1::sNluc5

    Plasmid

    #186762

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNHT7::UMSBP::sNluc2_ORF2

    Plasmid

    #186766

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNHT7::RPL15::sNluc2_STOP

    Plasmid

    #186767

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Premature stop codon at residue 16

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNHT7::RPL15::sGluc

    Plasmid

    #186769

    Purpose

    An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRps9-WT-IRES-GFP-HygR

    Plasmid

    #190271

    Purpose

    Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

    Depositor

    Erik Böttger

    Article

    Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

    Insert

    ribosomal protein RPS9
    

    Use

    Tags

    IRES-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS-CMV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRps9-D95N-IRES-GFP-HygR

    Plasmid

    #190272

    Purpose

    Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

    Depositor

    Erik Böttger

    Article

    Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

    Insert

    ribosomal protein RPS9
    

    Use

    Tags

    IRES-eGFP

    Expression

    Mammalian

    Mutation

    D95N

    Promoter

    CAGGS-CMV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET15b MOSPD2 RD/LD [282-490]

    Plasmid

    #186470

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT22gCfpUbiG4FER4P2A(#249)

    Plasmid

    #184070

    Purpose

    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    Gal4bdFF-ERT2-P2A
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Slc1a3-CreERT2 Targeting Vector

    Plasmid

    #129409

    Purpose

    Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

    Insert

    Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px335 Slc1a3 nickase 1

    Plasmid

    #129410

    Purpose

    Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

    Insert

    sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. px335 Slc1a3 nickase 2

    Plasmid

    #129411

    Purpose

    Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

    Insert

    sgRNA targeting Slc1a3 (Slc1a3 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge